6UTV

E. coli sigma-S transcription initiation complex with a 6-nt RNA ("Fresh" crystal soaked with CTP, UTP, GTP, and ddATP for 150 seconds)

これはPDB形式変換不可エントリーです。

6UTV の概要

• エントリーDOI: 10.2210/pdb6utv/pdb
• 分子名称: DNA-directed RNA polymerase subunit alpha, ZINC ION, DIPHOSPHATE, ... (11 entities in total)
• 機能のキーワード: transcription initiation, rna polymerase, dna promoter, transcription bubble, de novo rna synthesis, dna scrunching, sigma-s factor, transcription, transferase-dna-rna complex, transferase/dna/rna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 442165.04

構造登録者

Zuo, Y.,De, S.,Steitz, T.A. (登録日: 2019-10-30, 公開日: 2020-08-26, 最終更新日: 2023-10-11)

主引用文献

Zuo, Y.,De, S.,Feng, Y.,Steitz, T.A.
Structural Insights into Transcription Initiation from De Novo RNA Synthesis to Transitioning into Elongation.
Iscience, 23:101445-101445, 2020

PubMed Abstract: In bacteria, the dissociable σ subunit of the RNA polymerase (RNAP) is responsible for initiating RNA synthesis from specific DNA sites. As nascent RNA grows, downstream DNA unwinds and is pulled into the RNAP, causing stress accumulation and initiation complex destabilization. Processive transcription elongation requires at least partial separation of the σ factor from the RNAP core enzyme. Here, we present a series of transcription complexes captured between the early initiation and elongation phases via in-crystal RNA synthesis and cleavage. Crystal structures of these complexes indicate that stress accumulation during transcription initiation is not due to clashing of the growing nascent RNA with the σ loop, but results from scrunching of the template strand DNA that is contained inside the RNAP by the σ domain. Our results shed light on how scrunching of template-strand DNA drives both abortive initiation and σ-RNAP core separation to transition transcription from initiation to elongation.

PubMed: 32829286
DOI: 10.1016/j.isci.2020.101445

実験手法

X-RAY DIFFRACTION (3.45 Å)