6USO

Telomerase Reverse Transcriptase prenucleotide binary complex, TERT:DNA

6USO の概要

• エントリーDOI: 10.2210/pdb6uso/pdb
• 分子名称: Telomerase reverse transcriptase, DNA (5'-D(*GP*GP*TP*CP*AP*GP*GP*TP*CP*AP*GP*GP*TP*CP*A)-3'), DNA/RNA (5'-R(*CP*UP*GP*AP*CP*CP*UP*GP*AP*C)-D(P*CP*T)-R(P*GP*AP*C)-D(P*C)-3'), ... (5 entities in total)
• 機能のキーワード: telomerase, reverse transcriptase, polymerase, nuclear protein, transferase-rna-dna complex, transferase/rna/dna
• 由来する生物種: Tribolium castaneum (Red flour beetle); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 80416.39

構造登録者

Schaich, M.A.,Freudenthal, B.D. (登録日: 2019-10-28, 公開日: 2020-06-17, 最終更新日: 2023-10-11)

主引用文献

Schaich, M.A.,Sanford, S.L.,Welfer, G.A.,Johnson, S.A.,Khoang, T.H.,Opresko, P.L.,Freudenthal, B.D.
Mechanisms of nucleotide selection by telomerase.
Elife, 9:-, 2020

PubMed Abstract: Telomerase extends telomere sequences at chromosomal ends to protect genomic DNA. During this process it must select the correct nucleotide from a pool of nucleotides with various sugars and base pairing properties, which is critically important for the proper capping of telomeric sequences by shelterin. Unfortunately, how telomerase selects correct nucleotides is unknown. Here, we determined structures of telomerase reverse transcriptase (TERT) throughout its catalytic cycle and mapped the active site residues responsible for nucleoside selection, metal coordination, triphosphate binding, and RNA template stabilization. We found that TERT inserts a mismatch or ribonucleotide ~1 in 10,000 and ~1 in 14,000 insertion events, respectively. At biological ribonucleotide concentrations, these rates translate to ~40 ribonucleotides inserted per 10 kilobases. Human telomerase assays determined a conserved tyrosine steric gate regulates ribonucleotide insertion into telomeres. Cumulatively, our work provides insight into how telomerase selects the proper nucleotide to maintain telomere integrity.

PubMed: 32501800
DOI: 10.7554/eLife.55438

実験手法

X-RAY DIFFRACTION (2.54 Å)