6US4
MTH1 in complex with compound 32
Summary for 6US4
| Entry DOI | 10.2210/pdb6us4/pdb |
| Descriptor | 7,8-dihydro-8-oxoguanine triphosphatase, 5-(2,3-dichlorophenyl)[1,2,4]triazolo[1,5-a]pyridin-2-amine (3 entities in total) |
| Functional Keywords | hydrolase, nudt1, nudix hydrolase, inhibitor, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 1 |
| Total formula weight | 18532.86 |
| Authors | Newby, Z.E.R.,Lansdon, E.B. (deposition date: 2019-10-24, release date: 2020-04-01, Last modification date: 2023-10-11) |
| Primary citation | Farand, J.,Kropf, J.E.,Blomgren, P.,Xu, J.,Schmitt, A.C.,Newby, Z.E.,Wang, T.,Murakami, E.,Barauskas, O.,Sudhamsu, J.,Feng, J.Y.,Niedziela-Majka, A.,Schultz, B.E.,Schwartz, K.,Viatchenko-Karpinski, S.,Kornyeyev, D.,Kashishian, A.,Fan, P.,Chen, X.,Lansdon, E.B.,Ports, M.O.,Currie, K.S.,Watkins, W.J.,Notte, G.T. Discovery of Potent and Selective MTH1 Inhibitors for Oncology: Enabling Rapid Target (In)Validation. Acs Med.Chem.Lett., 11:358-364, 2020 Cited by PubMed Abstract: We describe the discovery of three structurally differentiated potent and selective MTH1 inhibitors and their subsequent use to investigate MTH1 as an oncology target, culminating in target (in)validation. Tetrahydronaphthyridine was rapidly identified as a highly potent MTH1 inhibitor (IC = 0.043 nM). Cocrystallization of with MTH1 revealed the ligand in a Φ---(pyridin-2-yl)acetamide conformation enabling a key intramolecular hydrogen bond and polar interactions with residues Gly34 and Asp120. Modification of literature compound with - and -linked aryl and alkyl aryl substituents led to the discovery of potent pyrimidine-2,4,6-triamine (IC = 0.49 nM). Triazolopyridine emerged as a highly selective lead compound with a suitable profile and desirable pharmacokinetic properties in rat. Elucidation of the DNA damage response, cell viability, and intracellular concentrations of oxo-NTPs (oxidized nucleoside triphosphates) as a function of MTH1 knockdown and/or small molecule inhibition was studied. Based on our findings, we were unable to provide evidence to further pursue MTH1 as an oncology target. PubMed: 32184970DOI: 10.1021/acsmedchemlett.9b00420 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.95032907402 Å) |
Structure validation
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