6US4

MTH1 in complex with compound 32

6US4 の概要

• エントリーDOI: 10.2210/pdb6us4/pdb
• 分子名称: 7,8-dihydro-8-oxoguanine triphosphatase, 5-(2,3-dichlorophenyl)[1,2,4]triazolo[1,5-a]pyridin-2-amine (3 entities in total)
• 機能のキーワード: hydrolase, nudt1, nudix hydrolase, inhibitor, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18532.86

構造登録者

Newby, Z.E.R.,Lansdon, E.B. (登録日: 2019-10-24, 公開日: 2020-04-01, 最終更新日: 2023-10-11)

主引用文献

Farand, J.,Kropf, J.E.,Blomgren, P.,Xu, J.,Schmitt, A.C.,Newby, Z.E.,Wang, T.,Murakami, E.,Barauskas, O.,Sudhamsu, J.,Feng, J.Y.,Niedziela-Majka, A.,Schultz, B.E.,Schwartz, K.,Viatchenko-Karpinski, S.,Kornyeyev, D.,Kashishian, A.,Fan, P.,Chen, X.,Lansdon, E.B.,Ports, M.O.,Currie, K.S.,Watkins, W.J.,Notte, G.T.
Discovery of Potent and Selective MTH1 Inhibitors for Oncology: Enabling Rapid Target (In)Validation.
Acs Med.Chem.Lett., 11:358-364, 2020

PubMed Abstract: We describe the discovery of three structurally differentiated potent and selective MTH1 inhibitors and their subsequent use to investigate MTH1 as an oncology target, culminating in target (in)validation. Tetrahydronaphthyridine was rapidly identified as a highly potent MTH1 inhibitor (IC = 0.043 nM). Cocrystallization of with MTH1 revealed the ligand in a Φ---(pyridin-2-yl)acetamide conformation enabling a key intramolecular hydrogen bond and polar interactions with residues Gly34 and Asp120. Modification of literature compound with - and -linked aryl and alkyl aryl substituents led to the discovery of potent pyrimidine-2,4,6-triamine (IC = 0.49 nM). Triazolopyridine emerged as a highly selective lead compound with a suitable profile and desirable pharmacokinetic properties in rat. Elucidation of the DNA damage response, cell viability, and intracellular concentrations of oxo-NTPs (oxidized nucleoside triphosphates) as a function of MTH1 knockdown and/or small molecule inhibition was studied. Based on our findings, we were unable to provide evidence to further pursue MTH1 as an oncology target.

PubMed: 32184970
DOI: 10.1021/acsmedchemlett.9b00420

実験手法

X-RAY DIFFRACTION (1.95032907402 Å)