6UMP

Crystal structure of MavC in complex with substrate mimic in P65 space group

6UMP の概要

• エントリーDOI: 10.2210/pdb6ump/pdb
• 分子名称: Ubiquitin, MavC, Ubiquitin-conjugating enzyme E2 N (3 entities in total)
• 機能のキーワード: legionella, effector, transglutaminase, ubiquitination, complex, transferase
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 69975.64

構造登録者

Puvar, K.,Iyer, S.,Luo, Z.Q.,Das, C. (登録日: 2019-10-10, 公開日: 2020-05-27, 最終更新日: 2024-10-23)

主引用文献

Puvar, K.,Iyer, S.,Fu, J.,Kenny, S.,Negron Teron, K.I.,Luo, Z.Q.,Brzovic, P.S.,Klevit, R.E.,Das, C.
Legionella effector MavC targets the Ube2N~Ub conjugate for noncanonical ubiquitination.
Nat Commun, 11:2365-2365, 2020

PubMed Abstract: The bacterial effector MavC modulates the host immune response by blocking Ube2N activity employing an E1-independent ubiquitin ligation, catalyzing formation of a γ-glutamyl-ε-Lys (Gln40-Lys92) isopeptide crosslink using a transglutaminase mechanism. Here we provide biochemical evidence in support of MavC targeting the activated, thioester-linked Ube2N~ubiquitin conjugate, catalyzing an intramolecular transglutamination reaction, covalently crosslinking the Ube2N and Ub subunits effectively inactivating the E2~Ub conjugate. Ubiquitin exhibits weak binding to MavC alone, but shows an increase in affinity when tethered to Ube2N in a disulfide-linked substrate that mimics the charged E2~Ub conjugate. Crystal structures of MavC in complex with the substrate mimic and crosslinked product provide insights into the reaction mechanism and underlying protein dynamics that favor transamidation over deamidation, while revealing a crucial role for the structurally unique insertion domain in substrate recognition. This work provides a structural basis of ubiquitination by transglutamination and identifies this enzyme's true physiological substrate.

PubMed: 32398758
DOI: 10.1038/s41467-020-16211-x

実験手法

X-RAY DIFFRACTION (2.8 Å)