6UJ6

X-ray Crystal Structure of Chromium-transferrin with Synergistic Anion Malonate

6UJ6 の概要

• エントリーDOI: 10.2210/pdb6uj6/pdb
• 分子名称: Transferrin, CHROMIUM ION, MALONATE ION, ... (6 entities in total)
• 機能のキーワード: chromium, transferrin, human, metal transport
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 77463.05

構造登録者

Petersen, C.M.,Edwards, K.C.,Gilbert, N.C.,Vincent, J.B.,Thompson, M.K. (登録日: 2019-10-02, 公開日: 2020-09-30, 最終更新日: 2024-10-30)

主引用文献

Petersen, C.M.,Edwards, K.C.,Gilbert, N.C.,Vincent, J.B.,Thompson, M.K.
X-ray structure of chromium(III)-containing transferrin: First structure of a physiological Cr(III)-binding protein.
J.Inorg.Biochem., 210:111101-111101, 2020

PubMed Abstract: Transferrin, the Fe(III) transport protein in mammalian blood, has been suggested to also serve as a Cr(III) transporter and as part of a Cr(III) detoxification system; however, the structure of the metal-binding sites has never been fully elucidated with bound Cr(III). Chromium(III)-transferrin was crystallized in the presence of the synergistic anion malonate. In the crystals, the protein exists with a closed C-terminal lobe containing a Cr(III) ion and an open, unoccupied N-terminal lobe. The overall structure and the metal ion environments are extremely similar to those of Fe(III)- and Ti(IV)-containing transferrin crystallized under comparable conditions. The octahedral coordination about the Cr(III) is comprised of four ligands provided by the protein (two tyrosine residues, a histidine residue, and an aspartate residue) and a chelating malonate anion. This represents the first crystal structure of a Cr(III)-containing protein that binds Cr(III) as part of its physiological function.

PubMed: 32650146
DOI: 10.1016/j.jinorgbio.2020.111101

実験手法

X-RAY DIFFRACTION (2.68 Å)