6UIU

Artificial Iron Proteins: Modelling the Active Sites in Non-Heme Dioxygenases

6UIU の概要

• エントリーDOI: 10.2210/pdb6uiu/pdb
• 分子名称: Streptavidin, N-(2-{bis[(pyridin-2-yl)methyl]amino}ethyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide (3 entities in total)
• 機能のキーワード: biotin binding artificial metalloprotein, metal binding protein
• 由来する生物種: Streptomyces avidinii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17021.58

構造登録者

Miller, K.R.,Paretsky, J.D.,Follmer, A.H.,Heinisch, T.,Mittra, K.,Gul, S.,Kim, I.-S.,Fuller, F.D.,Batyuk, A.,Sutherlin, K.D.,Brewster, A.S.,Bhowmick, A.,Sauter, N.K.,Kern, J.,Yano, J.,Green, M.T.,Ward, T.R.,Borovik, A.S. (登録日: 2019-10-01, 公開日: 2020-05-06, 最終更新日: 2023-10-11)

主引用文献

Miller, K.R.,Paretsky, J.D.,Follmer, A.H.,Heinisch, T.,Mittra, K.,Gul, S.,Kim, I.S.,Fuller, F.D.,Batyuk, A.,Sutherlin, K.D.,Brewster, A.S.,Bhowmick, A.,Sauter, N.K.,Kern, J.,Yano, J.,Green, M.T.,Ward, T.R.,Borovik, A.S.
Artificial Iron Proteins: Modeling the Active Sites in Non-Heme Dioxygenases.
Inorg.Chem., 59:6000-6009, 2020

PubMed Abstract: An important class of non-heme dioxygenases contains a conserved Fe binding site that consists of a 2-His-1-carboxylate facial triad. Results from structural biology show that, in the resting state, these proteins are six-coordinate with aqua ligands occupying the remaining three coordination sites. We have utilized biotin-streptavidin (Sav) technology to design new artificial Fe proteins (ArMs) that have many of the same structural features found within active sites of these non-heme dioxygenases. An Sav variant was isolated that contains the SE mutation, which installed a carboxylate side chain in the appropriate position to bind to a synthetic Fe complex confined within Sav. Structural studies using X-ray diffraction (XRD) methods revealed a facial triad binding site that is composed of two N donors from the biotinylated ligand and the monodentate coordination of the carboxylate from SE. Two aqua ligands complete the primary coordination sphere of the Fe center with both involved in hydrogen bond networks within Sav. The corresponding Fe protein was also prepared and structurally characterized to show a six-coordinate complex with two exogenous acetato ligands. The Fe protein was further shown to bind an exogenous azido ligand through replacement of one acetato ligand. Spectroscopic studies of the ArMs in solution support the results found by XRD.

PubMed: 32309932
DOI: 10.1021/acs.inorgchem.9b03791

実験手法

X-RAY DIFFRACTION (1.35 Å)