6UHJ

X-ray Structure of C148 mGFP

6UHJ の概要

• エントリーDOI: 10.2210/pdb6uhj/pdb
• 分子名称: C148 mGFP (2 entities in total)
• 機能のキーワード: green fluorescent protein, beta-barrel, fluorescent protein
• 由来する生物種: Aequorea victoria
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31038.91

構造登録者

Winegar, P.W.,Hayes, O.G.,McMillan, J.R.,Figg, C.A.,Focia, P.J.,Mirkin, C.A. (登録日: 2019-09-27, 公開日: 2020-03-18, 最終更新日: 2024-10-30)

主引用文献

Winegar, P.H.,Hayes, O.G.,McMillan, J.R.,Figg, C.A.,Focia, P.J.,Mirkin, C.A.
DNA-Directed Protein Packing within Single Crystals.
Chem, 6:1007-1017, 2020

PubMed Abstract: Designed DNA-DNA interactions are investigated for their ability to modulate protein packing within single crystals of mutant green fluorescent proteins (mGFPs) functionalized with a single DNA strand (mGFP-DNA). We probe the effects of DNA sequence, length, and protein-attachment position on the formation and protein packing of mGFP-DNA crystals. Notably, when complementary mGFP-DNA conjugates are introduced to one another, crystals form with nearly identical packing parameters, regardless of sequence if the number of bases is equivalent. DNA complementarity is essential, because experiments with non-complementary sequences produce crystals with different protein arrangements. Importantly, the DNA length and its position of attachment on the protein markedly influence the formation of and protein packing within single crystals. This work shows how designed DNA interactions can be used to influence the growth and packing in X-ray diffraction quality protein single crystals and is thus an important step forward in protein crystal engineering.

PubMed: 33709040
DOI: 10.1016/j.chempr.2020.03.002

実験手法

X-RAY DIFFRACTION (1.5 Å)