6UF2

NMR structure of biofilm-related Se0862 from Synechococcus elongatus

Summary for 6UF2

• Entry DOI: 10.2210/pdb6uf2/pdb
• NMR Information: BMRB: 30676
• Descriptor: Biofilm-related protein (1 entity in total)
• Functional Keywords: secretion, biofilm, unknown function
• Biological source: Synechococcus elongatus (strain PCC 7942)
• Total number of polymer chains: 1
• Total formula weight: 14425.34

Authors

Zhang, N.,LiWang, A.L. (deposition date: 2019-09-23, release date: 2020-09-30, Last modification date: 2024-05-15)

Primary citation

Zhang, N.,Chang, Y.G.,Tseng, R.,Ovchinnikov, S.,Schwarz, R.,LiWang, A.
Solution NMR structure of Se0862, a highly conserved cyanobacterial protein involved in biofilm formation.
Protein Sci., 29:2274-2280, 2020

PubMed Abstract: Biofilms are accumulations of microorganisms embedded in extracellular matrices that protect against external factors and stressful environments. Cyanobacterial biofilms are ubiquitous and have potential for treatment of wastewater and sustainable production of biofuels. But the underlying mechanisms regulating cyanobacterial biofilm formation are unclear. Here, we report the solution NMR structure of a protein, Se0862, conserved across diverse cyanobacterial species and involved in regulation of biofilm formation in the cyanobacterium Synechococcus elongatus PCC 7942. Se0862 is a class α+β protein with ααββββαα topology and roll architecture, consisting of a four-stranded β-sheet that is flanked by four α-helices on one side. Conserved surface residues constitute a hydrophobic pocket and charged regions that are likely also present in Se0862 orthologs.

PubMed: 32949024
DOI: 10.1002/pro.3952

Experimental method

SOLUTION NMR