6UDA

Cryo-EM structure of CH235UCA bound to Man5-enriched CH505.N279K.G458Y.SOSIP.664

6UDA の概要

• エントリーDOI: 10.2210/pdb6uda/pdb
• EMDBエントリー: 20735
• 分子名称: CH505.N279K.G458Y.SOSIP.664 gp120, CH235 UCA heavy chain Fab, CH505.N279K.G458Y.SOSIP.664 gp41, ... (6 entities in total)
• 機能のキーワード: ch235 lineage, ch505 virus, vaccine design, cryo-em, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 378539.12

構造登録者

Henderson, R.,Acharya, P. (登録日: 2019-09-19, 公開日: 2019-10-02, 最終更新日: 2024-10-30)

主引用文献

LaBranche, C.C.,Henderson, R.,Hsu, A.,Behrens, S.,Chen, X.,Zhou, T.,Wiehe, K.,Saunders, K.O.,Alam, S.M.,Bonsignori, M.,Borgnia, M.J.,Sattentau, Q.J.,Eaton, A.,Greene, K.,Gao, H.,Liao, H.X.,Williams, W.B.,Peacock, J.,Tang, H.,Perez, L.G.,Edwards, R.J.,Kepler, T.B.,Korber, B.T.,Kwong, P.D.,Mascola, J.R.,Acharya, P.,Haynes, B.F.,Montefiori, D.C.
Neutralization-guided design of HIV-1 envelope trimers with high affinity for the unmutated common ancester of CH235 lineage CD4bs broadly neutralizing antibodies.
Plos Pathog., 15:e1008026-e1008026, 2019

PubMed Abstract: The CD4 binding site (CD4bs) of the HIV-1 envelope glycoprotein is susceptible to multiple lineages of broadly neutralizing antibodies (bnAbs) that are attractive to elicit with vaccines. The CH235 lineage (VH1-46) of CD4bs bnAbs is particularly attractive because the most mature members neutralize 90% of circulating strains, do not possess long HCDR3 regions, and do not contain insertions and deletions that may be difficult to induce. We used virus neutralization to measure the interaction of CH235 unmutated common ancestor (CH235 UCA) with functional Env trimers on infectious virions to guide immunogen design for this bnAb lineage. Two Env mutations were identified, one in loop D (N279K) and another in V5 (G458Y), that acted synergistically to render autologous CH505 transmitted/founder virus susceptible to neutralization by CH235 UCA. Man5-enriched N-glycans provided additional synergy for neutralization. CH235 UCA bound with nanomolar affinity to corresponding soluble native-like Env trimers as candidate immunogens. A cryo-EM structure of CH235 UCA bound to Man5-enriched CH505.N279K.G458Y.SOSIP.664 revealed interactions of the antibody light chain complementarity determining region 3 (CDR L3) with the engineered Env loops D and V5. These results demonstrate that virus neutralization can directly inform vaccine design and suggest a germline targeting and reverse engineering strategy to initiate and mature the CH235 bnAb lineage.

PubMed: 31527908
DOI: 10.1371/journal.ppat.1008026

実験手法

ELECTRON MICROSCOPY (4.2 Å)