6U95

Adeno-associated virus strain AAVhu.37 capsid icosahedral structure

6U95 の概要

• エントリーDOI: 10.2210/pdb6u95/pdb
• EMDBエントリー: 20693
• 分子名称: Capsid protein VP1 (1 entity in total)
• 機能のキーワード: capsid, blood-brain barrier penetration, virus
• 由来する生物種: Adeno-associated virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 81528.85

構造登録者

Kaelber, J.T.,Yost, S.A.,Firlar, E.,Mercer, A.C. (登録日: 2019-09-06, 公開日: 2019-09-18, 最終更新日: 2024-03-20)

主引用文献

Kaelber, J.T.,Yost, S.A.,Webber, K.A.,Firlar, E.,Liu, Y.,Danos, O.,Mercer, A.C.
Structure of the AAVhu.37 capsid by cryoelectron microscopy.
Acta Crystallogr.,Sect.F, 76:58-64, 2020

PubMed Abstract: Adeno-associated viruses (AAVs) are used as in vivo gene-delivery vectors in gene-therapy products and have been heavily investigated for numerous indications. Over 100 naturally occurring AAV serotypes and variants have been isolated from primate samples. Many reports have described unique properties of these variants (for instance, differences in potency, target cell or evasion of the immune response), despite high amino-acid sequence conservation. AAVhu.37 is of interest for clinical applications owing to its proficient transduction of the liver and central nervous system. The sequence identity of the AAVhu.37 VP1 to the well characterized AAVrh.10 serotype, for which no structure is available, is greater than 98%. Here, the structure of the AAVhu.37 capsid at 2.56 Å resolution obtained via single-particle cryo-electron microscopy is presented.

PubMed: 32039886
DOI: 10.1107/S2053230X20000308

実験手法

ELECTRON MICROSCOPY (2.56 Å)