6U7Z

RNA hairpin structure containing one TNA nucleotide as primer

6U7Z の概要

• エントリーDOI: 10.2210/pdb6u7z/pdb
• 分子名称: RNA (5'-R(*CP*UP*GP*CP*UP*GP*GP*CP*UP*AP*AP*GP*GP*GP*CP*CP*GP*AP*AP*AP*GP*(TG))-3'), RNA (5'-R(*CP*UP*AP*UP*GP*CP*CP*UP*GP*CP*UP*G)-3'), CYTIDINE-5'-MONOPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: rna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 11209.75

構造登録者

Szostak, J.W.,Zhang, W. (登録日: 2019-09-03, 公開日: 2020-12-09, 最終更新日: 2024-07-03)

主引用文献

Zhang, W.,Kim, S.C.,Tam, C.P.,Lelyveld, V.S.,Bala, S.,Chaput, J.C.,Szostak, J.W.
Structural interpretation of the effects of threo-nucleotides on nonenzymatic template-directed polymerization.
Nucleic Acids Res., 49:646-656, 2021

PubMed Abstract: The prebiotic synthesis of ribonucleotides is likely to have been accompanied by the synthesis of noncanonical nucleotides including the threo-nucleotide building blocks of TNA. Here, we examine the ability of activated threo-nucleotides to participate in nonenzymatic template-directed polymerization. We find that primer extension by multiple sequential threo-nucleotide monomers is strongly disfavored relative to ribo-nucleotides. Kinetic, NMR and crystallographic studies suggest that this is due in part to the slow formation of the imidazolium-bridged TNA dinucleotide intermediate in primer extension, and in part because of the greater distance between the attacking RNA primer 3'-hydroxyl and the phosphate of the incoming threo-nucleotide intermediate. Even a single activated threo-nucleotide in the presence of an activated downstream RNA oligonucleotide is added to the primer 10-fold more slowly than an activated ribonucleotide. In contrast, a single activated threo-nucleotide at the end of an RNA primer or in an RNA template results in only a modest decrease in the rate of primer extension, consistent with the minor and local structural distortions revealed by crystal structures. Our results are consistent with a model in which heterogeneous primordial oligonucleotides would, through cycles of replication, have given rise to increasingly homogeneous RNA strands.

PubMed: 33347562
DOI: 10.1093/nar/gkaa1215

実験手法

X-RAY DIFFRACTION (2.71 Å)