6U7A

Rv3722c in complex with kynurenine

6U7A の概要

• エントリーDOI: 10.2210/pdb6u7a/pdb
• 分子名称: Aminotransferase, (2S)-4-(2-aminophenyl)-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]-4-oxobutanoic acid, PHOSPHATE ION, ... (9 entities in total)
• 機能のキーワード: aminotransferase, mycobacterium tuberculosis, transferase
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 384702.59

構造登録者

Mandyoli, L.,Sacchettini, J. (登録日: 2019-09-01, 公開日: 2020-05-06, 最終更新日: 2023-10-11)

主引用文献

Jansen, R.S.,Mandyoli, L.,Hughes, R.,Wakabayashi, S.,Pinkham, J.T.,Selbach, B.,Guinn, K.M.,Rubin, E.J.,Sacchettini, J.C.,Rhee, K.Y.
Aspartate aminotransferase Rv3722c governs aspartate-dependent nitrogen metabolism in Mycobacterium tuberculosis.
Nat Commun, 11:1960-1960, 2020

PubMed Abstract: Gene rv3722c of Mycobacterium tuberculosis is essential for in vitro growth, and encodes a putative pyridoxal phosphate-binding protein of unknown function. Here we use metabolomic, genetic and structural approaches to show that Rv3722c is the primary aspartate aminotransferase of M. tuberculosis, and mediates an essential but underrecognized role in metabolism: nitrogen distribution. Rv3722c deficiency leads to virulence attenuation in macrophages and mice. Our results identify aspartate biosynthesis and nitrogen distribution as potential species-selective drug targets in M. tuberculosis.

PubMed: 32327655
DOI: 10.1038/s41467-020-15876-8

実験手法

X-RAY DIFFRACTION (2.22 Å)