6U57

KDO8PS Structure Determined at the EuXFEL using Segmented Flow Injection

6U57 の概要

• エントリーDOI: 10.2210/pdb6u57/pdb
• 分子名称: 2-dehydro-3-deoxyphosphooctonate aldolase (2 entities in total)
• 機能のキーワード: catalytic activity, transferase activity, transferase
• 由来する生物種: Escherichia coli (strain B / BL21-DE3)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30870.68

構造登録者

Botha, S.,Ros, A. (登録日: 2019-08-27, 公開日: 2020-09-02, 最終更新日: 2023-10-11)

主引用文献

Echelmeier, A.,Cruz Villarreal, J.,Messerschmidt, M.,Kim, D.,Coe, J.D.,Thifault, D.,Botha, S.,Egatz-Gomez, A.,Gandhi, S.,Brehm, G.,Conrad, C.E.,Hansen, D.T.,Madsen, C.,Bajt, S.,Meza-Aguilar, J.D.,Oberthur, D.,Wiedorn, M.O.,Fleckenstein, H.,Mendez, D.,Knoska, J.,Martin-Garcia, J.M.,Hu, H.,Lisova, S.,Allahgholi, A.,Gevorkov, Y.,Ayyer, K.,Aplin, S.,Ginn, H.M.,Graafsma, H.,Morgan, A.J.,Greiffenberg, D.,Klujev, A.,Laurus, T.,Poehlsen, J.,Trunk, U.,Mezza, D.,Schmidt, B.,Kuhn, M.,Fromme, R.,Sztuk-Dambietz, J.,Raab, N.,Hauf, S.,Silenzi, A.,Michelat, T.,Xu, C.,Danilevski, C.,Parenti, A.,Mekinda, L.,Weinhausen, B.,Mills, G.,Vagovic, P.,Kim, Y.,Kirkwood, H.,Bean, R.,Bielecki, J.,Stern, S.,Giewekemeyer, K.,Round, A.R.,Schulz, J.,Dorner, K.,Grant, T.D.,Mariani, V.,Barty, A.,Mancuso, A.P.,Weierstall, U.,Spence, J.C.H.,Chapman, H.N.,Zatsepin, N.,Fromme, P.,Kirian, R.A.,Ros, A.
Segmented flow generator for serial crystallography at the European X-ray free electron laser.
Nat Commun, 11:4511-4511, 2020

PubMed Abstract: Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuously jets the protein crystal suspension into the path of the XFEL, wasting a vast amount of sample due to the pulsed nature of all current XFEL sources. The European XFEL (EuXFEL) delivers femtosecond (fs) X-ray pulses in trains spaced 100 ms apart whereas pulses within trains are currently separated by 889 ns. Therefore, continuous sample delivery via fast jets wastes >99% of sample. Here, we introduce a microfluidic device delivering crystal laden droplets segmented with an immiscible oil reducing sample waste and demonstrate droplet injection at the EuXFEL compatible with high pressure liquid delivery of an SFX experiment. While achieving ~60% reduction in sample waste, we determine the structure of the enzyme 3-deoxy-D-manno-octulosonate-8-phosphate synthase from microcrystals delivered in droplets revealing distinct structural features not previously reported.

PubMed: 32908128
DOI: 10.1038/s41467-020-18156-7

実験手法

X-RAY DIFFRACTION (2.8 Å)