6U4L

cysteine dioxygenase variant - C93E

6U4L の概要

• エントリーDOI: 10.2210/pdb6u4l/pdb
• 分子名称: Cysteine dioxygenase type 1, FE (III) ION, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: non-heme, dioxygenase, oxidoreductase
• 由来する生物種: Rattus norvegicus (Rat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23185.72

構造登録者

Meneely, K.M.,Chilton, A.S.,Forbes, D.L.,Ellis, H.R.,Lamb, A.L. (登録日: 2019-08-26, 公開日: 2020-07-08, 最終更新日: 2023-10-11)

主引用文献

Forbes, D.L.,Meneely, K.M.,Chilton, A.S.,Lamb, A.L.,Ellis, H.R.
The 3-His Metal Coordination Site Promotes the Coupling of Oxygen Activation to Cysteine Oxidation in Cysteine Dioxygenase.
Biochemistry, 59:2022-2031, 2020

PubMed Abstract: Cysteine dioxygenase (CDO) structurally resembles cupin enzymes that use a 3-His/1-Glu coordination scheme. However, the glutamate ligand is substituted with a cysteine (Cys93) residue, which forms a thioether bond with tyrosine (Tyr157) under physiological conditions. The reversion variant, C93E CDO, was generated in order to reestablish the more common 3-His/1-Glu metal ligands of the cupin superfamily. This variant provides a framework for testing the structural and functional significance of Cys93 and the cross-link in CDO. Although dioxygen consumption was observed with C93E CDO, it was not coupled with l-cysteine oxidation. Substrate analogues (d-cysteine, cysteamine, and 3-mercaptopropionate) were not viable substrates for the C93E CDO variant, although they showed variable coordinations to the iron center. The structures of C93E and cross-linked and non-cross-linked wild-type CDO were solved by X-ray crystallography to 1.91, 2.49, and 2.30 Å, respectively. The C93E CDO variant had similar overall structural properties compared to cross-linked CDO; however, the iron was coordinated by a 3-His/1-Glu geometry, leaving only two coordination sites available for dioxygen and bidentate l-cysteine binding. The hydroxyl group of Tyr157 shifted in both non-cross-linked and C93E CDO, and this displacement prevented the residue from participating in substrate stabilization. Based on these results, the divergence of the metal center of cysteine dioxygenase from the 3-His/1-Glu geometry seen with many cupin enzymes was essential for effective substrate binding. The substitution of Glu with Cys in CDO allows for a third coordination site on the iron for bidentate cysteine and monodentate oxygen binding.

PubMed: 32368901
DOI: 10.1021/acs.biochem.9b01085

実験手法

X-RAY DIFFRACTION (1.911 Å)