6U3U

Crystal Structure of Shiga Toxin 2K

Summary for 6U3U

• Entry DOI: 10.2210/pdb6u3u/pdb
• Descriptor: Shiga toxin 2K subunit A, Shiga toxin 2K subunit B, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ... (6 entities in total)
• Functional Keywords: shiga toxin, ab5 toxin, toxoid, toxin
• Biological source: Escherichia coli; More
• Total number of polymer chains: 12
• Total formula weight: 144540.26

Authors

Zhang, Y.Z.,He, X.H. (deposition date: 2019-08-22, release date: 2020-07-01, Last modification date: 2024-10-16)

Primary citation

Hughes, A.C.,Zhang, Y.,Bai, X.,Xiong, Y.,Wang, Y.,Yang, X.,Xu, Q.,He, X.
Structural and Functional Characterization of Stx2k, a New Subtype of Shiga Toxin 2.
Microorganisms, 8:-, 2019

PubMed Abstract: Shiga toxin (Stx) is the major virulence factor of Shiga toxin-producing (STEC). Stx evolves rapidly and, as such, new subtypes continue to emerge that challenge the efficacy of existing disease management and surveillance strategies. A new subtype, Stx2k, was recently identified in isolated from a wide range of sources including diarrheal patients, animals, and raw meats, and was poorly detected by existing immunoassays. In this study, the structure of Stx2kE167Q was determined at 2.29 Å resolution and the conservation of structure with Stx2a was revealed. A novel polyclonal antibody capable of neutralizing Stx2k and an immunoassay, with a 10-fold increase in sensitivity compared to assays using extant antibodies, were developed. Stx2k is less toxic than Stx2a in Vero cell assays but is similar to Stx2a in receptor-binding preference, thermostability, and acid tolerance. Although Stx2k does not appear to be as potent as Stx2a to Vero cells, the wide distribution and blended virulence profiles of the Stx2k-producing strains suggest that horizontal gene transfer through Stx2k-converting phages could result in the emergence of new and highly virulent pathogens. This study provides useful information and tools for early detection and control of Stx2k-producing , which could reduce public risk of infection by less-known STECs.

PubMed: 31861375
DOI: 10.3390/microorganisms8010004

Experimental method

X-RAY DIFFRACTION (2.287 Å)