6TZR

A DNA G-quadruplex/i-motif hybrid

6TZR の概要

• エントリーDOI: 10.2210/pdb6tzr/pdb
• 分子名称: DNA (5'-D(*CP*CP*AP*GP*GP*CP*(BRU)P*GP*CP*AP*A)-3'), BARIUM ION (2 entities in total)
• 機能のキーワード: g-quadruplex, i-motif, quadruplex, hybrid, non-duplex, noncanonical, dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6953.47

構造登録者

Chu, B.,Paukstelis, P.J. (登録日: 2019-08-13, 公開日: 2019-10-16, 最終更新日: 2024-03-13)

主引用文献

Chu, B.,Zhang, D.,Paukstelis, P.J.
A DNA G-quadruplex/i-motif hybrid.
Nucleic Acids Res., 47:11921-11930, 2019

PubMed Abstract: DNA can form many structures beyond the canonical Watson-Crick double helix. It is now clear that noncanonical structures are present in genomic DNA and have biological functions. G-rich G-quadruplexes and C-rich i-motifs are the most well-characterized noncanonical DNA motifs that have been detected in vivo with either proscribed or postulated biological roles. Because of their independent sequence requirements, these structures have largely been considered distinct types of quadruplexes. Here, we describe the crystal structure of the DNA oligonucleotide, d(CCAGGCTGCAA), that self-associates to form a quadruplex structure containing two central antiparallel G-tetrads and six i-motif C-C+ base pairs. Solution studies suggest a robust structural motif capable of assembling as a tetramer of individual strands or as a dimer when composed of tandem repeats. This hybrid structure highlights the growing structural diversity of DNA and suggests that biological systems may harbor many functionally important non-duplex structures.

PubMed: 31724696
DOI: 10.1093/nar/gkz1008

実験手法

X-RAY DIFFRACTION (2.4 Å)