6TZ0

In situ structure of BmCPV RNA-dependent RNA polymerase at abortive state

6TZ0 の概要

• エントリーDOI: 10.2210/pdb6tz0/pdb
• EMDBエントリー: 20581 20582 20585 20586 20587
• 分子名称: RNA-dependent RNA Polymerase, Viral structural protein 4 (2 entities in total)
• 機能のキーワード: rdrp, viral protein, transferase
• 由来する生物種: Bombyx mori cytoplasmic polyhedrosis virus (BmCPV); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 202690.86

構造登録者

Cui, Y.X.,Zhang, Y.N.,Sun, J.C.,Zhou, Z.H. (登録日: 2019-08-09, 公開日: 2019-11-20, 最終更新日: 2025-05-28)

主引用文献

Cui, Y.,Zhang, Y.,Zhou, K.,Sun, J.,Zhou, Z.H.
Conservative transcription in three steps visualized in a double-stranded RNA virus.
Nat.Struct.Mol.Biol., 26:1023-1034, 2019

PubMed Abstract: Endogenous RNA transcription characterizes double-stranded RNA (dsRNA) viruses in the Reoviridae, a family that is exemplified by its simple, single-shelled member cytoplasmic polyhedrosis virus (CPV). Because of the lack of in situ structures of the intermediate stages of RNA-dependent RNA polymerase (RdRp) during transcription, it is poorly understood how RdRp detects environmental cues and internal transcriptional states to initiate and coordinate repeated cycles of transcript production inside the capsid. Here, we captured five high-resolution (2.8-3.5 Å) RdRp-RNA in situ structures-representing quiescent, initiation, early elongation, elongation and abortive states-under seven experimental conditions of CPV. We observed the 'Y'-form initial RNA fork in the initiation state and the complete transcription bubble in the elongation state. These structures reveal that de novo RNA transcription involves three major conformational changes during state transitions. Our results support an ouroboros model for endogenous conservative transcription in dsRNA viruses.

PubMed: 31695188
DOI: 10.1038/s41594-019-0320-0

実験手法

ELECTRON MICROSCOPY (2.8 Å)