6TY3

FAK structure from single particle analysis of 2D crystals

6TY3 の概要

• エントリーDOI: 10.2210/pdb6ty3/pdb
• EMDBエントリー: 10615
• 分子名称: Focal adhesion kinase 1 (1 entity in total)
• 機能のキーワード: kinase, focal adhesion, membrane, cell adhesion
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 150952.89

構造登録者

Acebron, I.,Righetto, R.,Biyani, N.,Chami, M.,Boskovic, J.,Stahlberg, H.,Lietha, D. (登録日: 2020-01-15, 公開日: 2020-08-19, 最終更新日: 2024-05-22)

主引用文献

Acebron, I.,Righetto, R.D.,Schoenherr, C.,de Buhr, S.,Redondo, P.,Culley, J.,Rodriguez, C.F.,Daday, C.,Biyani, N.,Llorca, O.,Byron, A.,Chami, M.,Grater, F.,Boskovic, J.,Frame, M.C.,Stahlberg, H.,Lietha, D.
Structural basis of Focal Adhesion Kinase activation on lipid membranes.
Embo J., 39:e104743-e104743, 2020

PubMed Abstract: Focal adhesion kinase (FAK) is a key component of the membrane proximal signaling layer in focal adhesion complexes, regulating important cellular processes, including cell migration, proliferation, and survival. In the cytosol, FAK adopts an autoinhibited state but is activated upon recruitment into focal adhesions, yet how this occurs or what induces structural changes is unknown. Here, we employ cryo-electron microscopy to reveal how FAK associates with lipid membranes and how membrane interactions unlock FAK autoinhibition to promote activation. Intriguingly, initial binding of FAK to the membrane causes steric clashes that release the kinase domain from autoinhibition, allowing it to undergo a large conformational change and interact itself with the membrane in an orientation that places the active site toward the membrane. In this conformation, the autophosphorylation site is exposed and multiple interfaces align to promote FAK oligomerization on the membrane. We show that interfaces responsible for initial dimerization and membrane attachment are essential for FAK autophosphorylation and resulting cellular activity including cancer cell invasion, while stable FAK oligomerization appears to be needed for optimal cancer cell proliferation in an anchorage-independent manner. Together, our data provide structural details of a key membrane bound state of FAK that is primed for efficient autophosphorylation and activation, hence revealing the critical event in integrin mediated FAK activation and signaling at focal adhesions.

PubMed: 32779739
DOI: 10.15252/embj.2020104743

実験手法

ELECTRON MICROSCOPY (6.32 Å)