6TV0

Serratia spp. cyanase hydratase

6TV0 の概要

• エントリーDOI: 10.2210/pdb6tv0/pdb
• 分子名称: Cyanate hydratase, OXALIC ACID, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: enzyme, lyase, hydrolase
• 由来する生物種: Serratia sp.
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 174368.79

構造登録者

Pederzoli, R.,Tarantino, D.,Gourlay, L.J.,Chaves-Sanjuan, A.,Bolognesi, M. (登録日: 2020-01-08, 公開日: 2021-01-27, 最終更新日: 2024-01-24)

主引用文献

Pederzoli, R.,Tarantino, D.,Gourlay, L.J.,Chaves-Sanjuan, A.,Bolognesi, M.
Detecting the nature and solving the crystal structure of a contaminant protein from an opportunistic pathogen.
Acta Crystallogr.,Sect.F, 76:392-397, 2020

PubMed Abstract: The unintentional crystallization of contaminant proteins in the place of target recombinant proteins is sporadically reported, despite the availability of stringent expression/purification protocols and of software for the detection of contaminants. Typically, the contaminant protein originates from the expression organism (for example Escherichia coli), but in rare circumstances contaminants from different sources have been reported. Here, a case of contamination from a Serratia bacterial strain that occurred while attempting to crystallize an unrelated protein from Burkholderia pseudomallei (overexpressed in E. coli) is presented. The contamination led to the unintended crystallization and structure analysis of a cyanase hydratase from a bacterial strain of the Serratia genus, an opportunistic enterobacterium that grows under conditions similar to those of E. coli and that is found in a variety of habitats, including the laboratory environment. In this context, the procedures that were adopted to identify the contaminant based on crystallographic data only are presented and the crystal structure of Serrata spp. cyanase hydratase is briefly discussed.

PubMed: 32880586
DOI: 10.1107/S2053230X20010626

実験手法

X-RAY DIFFRACTION (1.96 Å)