6TRM

Solution structure of the antifungal protein PAFC

6TRM の概要

• エントリーDOI: 10.2210/pdb6trm/pdb
• NMR情報: BMRB: 34468
• 分子名称: Pc21g12970 protein (1 entity in total)
• 機能のキーワード: solution structure, disulphide protein, pafc, structure from cyana 2.1, antimicrobial protein
• 由来する生物種: Penicillium rubens Wisconsin 54-1255
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6639.28

構造登録者

Czajlik, A.,Holzknecht, J.,Marx, F.,Batta, G. (登録日: 2019-12-19, 公開日: 2020-10-28, 最終更新日: 2024-11-13)

主引用文献

Czajlik, A.,Holzknecht, J.,Galgoczy, L.,Toth, L.,Poor, P.,Ordog, A.,Varadi, G.,Kuhbacher, A.,Borics, A.,Toth, G.K.,Marx, F.,Batta, G.
Solution Structure, Dynamics, and New Antifungal Aspects of the Cysteine-Rich Miniprotein PAFC.
Int J Mol Sci, 22:-, 2021

PubMed Abstract: The genome of Q176 contains a gene coding for the 88-amino-acid (aa)-long glycine- and cysteine-rich antifungal protein C (PAFC). After maturation, the secreted antifungal miniprotein (MP) comprises 64 aa and shares 80% aa identity with the bubble protein (BP) from , which has a published X-ray structure. Our team expressed isotope (N, C)-labeled, recombinant PAFC in high yields, which allowed us to determine the solution structure and molecular dynamics by nuclear magnetic resonance (NMR) experiments. The primary structure of PAFC is dominated by 14 glycines, and therefore, whether the four disulfide bonds can stabilize the fold is challenging. Indeed, unlike the few published solution structures of other antifungal MPs from filamentous ascomycetes, the NMR data indicate that PAFC has shorter secondary structure elements and lacks the typical β-barrel structure, though it has a positively charged cavity and a hydrophobic core around the disulfide bonds. Some parts within the two putative γ-core motifs exhibited enhanced dynamics according to a new disorder index presentation of N-NMR relaxation data. Furthermore, we also provided a more detailed insight into the antifungal spectrum of PAFC, with specific emphasis on fungal plant pathogens. Our results suggest that PAFC could be an effective candidate for the development of new antifungal strategies in agriculture.

PubMed: 33504082
DOI: 10.3390/ijms22031183

実験手法

SOLUTION NMR