6TQF

The structure of ABC transporter Rv1819c in AMP-PNP bound state

6TQF の概要

• エントリーDOI: 10.2210/pdb6tqf/pdb
• 関連するPDBエントリー: 6TQE
• EMDBエントリー: 10550
• 分子名称: ABC transporter ATP-binding protein/permease, DODECYL-BETA-D-MALTOSIDE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: cobalamin, vitamin b12, abc transporter, exporter fold, import, tuberculosis, transport protein
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 151011.15

構造登録者

Rempel, S.,Gati, C.,Slotboom, D.J.,Guskov, A. (登録日: 2019-12-16, 公開日: 2020-04-01, 最終更新日: 2025-07-09)

主引用文献

Rempel, S.,Gati, C.,Nijland, M.,Thangaratnarajah, C.,Karyolaimos, A.,de Gier, J.W.,Guskov, A.,Slotboom, D.J.
A mycobacterial ABC transporter mediates the uptake of hydrophilic compounds.
Nature, 580:409-412, 2020

PubMed Abstract: Mycobacterium tuberculosis (Mtb) is an obligate human pathogen and the causative agent of tuberculosis. Although Mtb can synthesize vitamin B (cobalamin) de novo, uptake of cobalamin has been linked to pathogenesis of tuberculosis. Mtb does not encode any characterized cobalamin transporter; however, the gene rv1819c was found to be essential for uptake of cobalamin. This result is difficult to reconcile with the original annotation of Rv1819c as a protein implicated in the transport of antimicrobial peptides such as bleomycin. In addition, uptake of cobalamin seems inconsistent with the amino acid sequence, which suggests that Rv1819c has a bacterial ATP-binding cassette (ABC)-exporter fold. Here, we present structures of Rv1819c, which reveal that the protein indeed contains the ABC-exporter fold, as well as a large water-filled cavity of about 7,700 Å, which enables the protein to transport the unrelated hydrophilic compounds bleomycin and cobalamin. On the basis of these structures, we propose that Rv1819c is a multi-solute transporter for hydrophilic molecules, analogous to the multidrug exporters of the ABC transporter family, which pump out structurally diverse hydrophobic compounds from cells.

PubMed: 32296172
DOI: 10.1038/s41586-020-2072-8

実験手法

ELECTRON MICROSCOPY (3.5 Å)