6TPH
Structure of a protein-RNA complex by ssNMR
Summary for 6TPH
| Entry DOI | 10.2210/pdb6tph/pdb |
| NMR Information | BMRB: 34465 |
| Descriptor | 50S ribosomal protein L7Ae, RNA (26-MER) (2 entities in total) |
| Functional Keywords | solid-state rna, protein-rna complex, structure determination, pre, rna |
| Biological source | Pyrococcus furiosus More |
| Total number of polymer chains | 2 |
| Total formula weight | 21821.74 |
| Authors | Mumdooh, A.,Marchanka, A.,Carlomagno, T. (deposition date: 2019-12-13, release date: 2020-02-12, Last modification date: 2024-06-19) |
| Primary citation | Ahmed, M.,Marchanka, A.,Carlomagno, T. Structure of a Protein-RNA Complex by Solid-State NMR Spectroscopy. Angew.Chem.Int.Ed.Engl., 59:6866-6873, 2020 Cited by PubMed Abstract: Solid-state NMR (ssNMR) is applicable to high molecular-weight (MW) protein assemblies in a non-amorphous precipitate. The technique yields atomic resolution structural information on both soluble and insoluble particles without limitations of MW or requirement of crystals. Herein, we propose and demonstrate an approach that yields the structure of protein-RNA complexes (RNP) solely from ssNMR data. Instead of using low-sensitivity magnetization transfer steps between heteronuclei of the protein and the RNA, we measure paramagnetic relaxation enhancement effects elicited on the RNA by a paramagnetic tag coupled to the protein. We demonstrate that this data, together with chemical-shift-perturbation data, yields an accurate structure of an RNP complex, starting from the bound structures of its components. The possibility of characterizing protein-RNA interactions by ssNMR may enable applications to large RNP complexes, whose structures are not accessible by other methods. PubMed: 32023357DOI: 10.1002/anie.201915465 PDB entries with the same primary citation |
| Experimental method | SOLID-STATE NMR |
Structure validation
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