6TPH

Structure of a protein-RNA complex by ssNMR

6TPH の概要

• エントリーDOI: 10.2210/pdb6tph/pdb
• NMR情報: BMRB: 34465
• 分子名称: 50S ribosomal protein L7Ae, RNA (26-MER) (2 entities in total)
• 機能のキーワード: solid-state rna, protein-rna complex, structure determination, pre, rna
• 由来する生物種: Pyrococcus furiosus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21821.74

構造登録者

Mumdooh, A.,Marchanka, A.,Carlomagno, T. (登録日: 2019-12-13, 公開日: 2020-02-12, 最終更新日: 2024-06-19)

主引用文献

Ahmed, M.,Marchanka, A.,Carlomagno, T.
Structure of a Protein-RNA Complex by Solid-State NMR Spectroscopy.
Angew.Chem.Int.Ed.Engl., 59:6866-6873, 2020

PubMed Abstract: Solid-state NMR (ssNMR) is applicable to high molecular-weight (MW) protein assemblies in a non-amorphous precipitate. The technique yields atomic resolution structural information on both soluble and insoluble particles without limitations of MW or requirement of crystals. Herein, we propose and demonstrate an approach that yields the structure of protein-RNA complexes (RNP) solely from ssNMR data. Instead of using low-sensitivity magnetization transfer steps between heteronuclei of the protein and the RNA, we measure paramagnetic relaxation enhancement effects elicited on the RNA by a paramagnetic tag coupled to the protein. We demonstrate that this data, together with chemical-shift-perturbation data, yields an accurate structure of an RNP complex, starting from the bound structures of its components. The possibility of characterizing protein-RNA interactions by ssNMR may enable applications to large RNP complexes, whose structures are not accessible by other methods.

PubMed: 32023357
DOI: 10.1002/anie.201915465

実験手法

SOLID-STATE NMR