6TOB

Structural and DNA Binding Properties of Mycobacterial Integration Host Factor mIHF

6TOB の概要

• エントリーDOI: 10.2210/pdb6tob/pdb
• NMR情報: BMRB: 34463
• 分子名称: Integration host factor MIHF (1 entity in total)
• 機能のキーワード: - transcriptional control. mycobacterial integration host factor - mihf is an alpha-helical protein with two dna-binding sites, dna binding protein
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11909.89

構造登録者

Herrmann, T. (登録日: 2019-12-11, 公開日: 2019-12-25, 最終更新日: 2024-06-19)

主引用文献

Odermatt, N.T.,Lelli, M.,Herrmann, T.,Abriata, L.A.,Japaridze, A.,Voilquin, H.,Singh, R.,Piton, J.,Emsley, L.,Dietler, G.,Cole, S.T.
Structural and DNA binding properties of mycobacterial integration host factor mIHF.
J.Struct.Biol., 209:107434-107434, 2020

PubMed Abstract: In bacteria, nucleoid associated proteins (NAPs) take part in active chromosome organization by supercoil management, three-dimensional DNA looping and direct transcriptional control. Mycobacterial integration host factor (mIHF, rv1388) is a NAP restricted to Actinobacteria and essential for survival of the human pathogen Mycobacterium tuberculosis. We show in vitro that DNA binding by mIHF strongly stabilizes the protein and increases its melting temperature. The structure obtained by Nuclear Magnetic Resonance (NMR) spectroscopy characterizes mIHF as a globular protein with a protruding alpha helix and a disordered N-terminus, similar to Streptomyces coelicolor IHF (sIHF). NMR revealed no residues of high flexibility, suggesting that mIHF is a rigid protein overall that does not undergo structural rearrangements. We show that mIHF only binds to double stranded DNA in solution, through two DNA binding sites (DBSs) similar to those identified in the X-ray structure of sIHF. According to Atomic Force Microscopy, mIHF is able to introduce left-handed loops of ca. 100 nm size (~300 bp) in supercoiled cosmids, thereby unwinding and relaxing the DNA.

PubMed: 31846718
DOI: 10.1016/j.jsb.2019.107434

実験手法

SOLUTION NMR