6TNN

Mini-RNase III (Mini-III) bound to 50S ribosome with precursor 23S rRNA

6TNN の概要

• エントリーDOI: 10.2210/pdb6tnn/pdb
• EMDBエントリー: 10535
• 分子名称: 50S ribosomal protein L10, 50S ribosomal protein L13, 50S ribosomal protein L14, ... (32 entities in total)
• 機能のキーワード: mini-rnase iii, mini-iii, complex, ribosome, 50s, rna maturation, rna processing, precursor 23s rrna, pre-23s rrna, 23s rrna, l3, ribosomal protein, rnase iii-like fold, rnase
• 由来する生物種: Bacillus subtilis subsp. subtilis str. 168 ; 詳細
• タンパク質・核酸の鎖数: 31
• 化学式量合計: 1381499.64

構造登録者

Oerum, S.,Dendooven, T.,Gilet, L.,Catala, M.,Degut, C.,Trinquier, A.,Barraud, P.,Luisi, B.,Condon, C.,Tisne, C. (登録日: 2019-12-09, 公開日: 2020-09-30, 最終更新日: 2024-05-22)

主引用文献

Oerum, S.,Dendooven, T.,Catala, M.,Gilet, L.,Degut, C.,Trinquier, A.,Bourguet, M.,Barraud, P.,Cianferani, S.,Luisi, B.F.,Condon, C.,Tisne, C.
Structures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs.
Mol.Cell, 80:227-, 2020

PubMed Abstract: The pathways for ribosomal RNA (rRNA) maturation diverge greatly among the domains of life. In the Gram-positive model bacterium, Bacillus subtilis, the final maturation steps of the two large ribosomal subunit (50S) rRNAs, 23S and 5S pre-rRNAs, are catalyzed by the double-strand specific ribonucleases (RNases) Mini-RNase III and RNase M5, respectively. Here we present a protocol that allowed us to solve the 3.0 and 3.1 Å resolution cryoelectron microscopy structures of these RNases poised to cleave their pre-rRNA substrates within the B. subtilis 50S particle. These data provide the first structural insights into rRNA maturation in bacteria by revealing how these RNases recognize and process double-stranded pre-rRNA. Our structures further uncover how specific ribosomal proteins act as chaperones to correctly fold the pre-rRNA substrates and, for Mini-III, anchor the RNase to the ribosome. These r-proteins thereby serve a quality-control function in the process from accurate ribosome assembly to rRNA processing.

PubMed: 32991829
DOI: 10.1016/j.molcel.2020.09.008

実験手法

ELECTRON MICROSCOPY (3.07 Å)