6TLB

Plasmodium falciparum lipocalin (PF3D7_0925900)

6TLB の概要

• エントリーDOI: 10.2210/pdb6tlb/pdb
• 分子名称: Serine/threonine protein kinase, GLYCEROL, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: lipocalin, malaria, infection, unknown function
• 由来する生物種: Plasmodium falciparum (isolate 3D7)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 89402.13

構造登録者

Burda, P.C.,Crosskey, T.D.,Lauk, K.,Wilmanns, M.,Gilberger, T.W. (登録日: 2019-12-02, 公開日: 2020-06-24, 最終更新日: 2024-11-06)

主引用文献

Burda, P.C.,Crosskey, T.,Lauk, K.,Zurborg, A.,Sohnchen, C.,Liffner, B.,Wilcke, L.,Pietsch, E.,Strauss, J.,Jeffries, C.M.,Svergun, D.I.,Wilson, D.W.,Wilmanns, M.,Gilberger, T.W.
Structure-Based Identification and Functional Characterization of a Lipocalin in the Malaria Parasite Plasmodium falciparum.
Cell Rep, 31:107817-107817, 2020

PubMed Abstract: Proteins of the lipocalin family are known to bind small hydrophobic ligands and are involved in various physiological processes ranging from lipid transport to oxidative stress responses. The genome of the malaria parasite Plasmodium falciparum contains a single protein PF3D7_0925900 with a lipocalin signature. Using crystallography and small-angle X-ray scattering, we show that the protein has a tetrameric structure of typical lipocalin monomers; hence we name it P. falciparum lipocalin (PfLCN). We show that PfLCN is expressed in the intraerythrocytic stages of the parasite and localizes to the parasitophorous and food vacuoles. Conditional knockdown of PfLCN impairs parasite development, which can be rescued by treatment with the radical scavenger Trolox or by temporal inhibition of hemoglobin digestion. This suggests a key function of PfLCN in counteracting oxidative stress-induced cell damage during multiplication of parasites within erythrocytes.

PubMed: 32579913
DOI: 10.1016/j.celrep.2020.107817

実験手法

SOLUTION SCATTERING
X-RAY DIFFRACTION (2.85 Å)