6TL4

Photosensory module (PAS-GAF-PHY) of Glycine max phyB

Summary for 6TL4

• Entry DOI: 10.2210/pdb6tl4/pdb
• Descriptor: Phytochrome, PHYCOCYANOBILIN (3 entities in total)
• Functional Keywords: phytochrome, plant protein
• Biological source: Glycine max (Soybean)
• Total number of polymer chains: 1
• Total formula weight: 60484.96

Authors

Nagano, S.,Guan, K.,Shenkutie, S.M.,Hughes, J.E. (deposition date: 2019-12-01, release date: 2020-05-06, Last modification date: 2024-01-24)

Primary citation

Nagano, S.,Guan, K.,Shenkutie, S.M.,Feiler, C.,Weiss, M.,Kraskov, A.,Buhrke, D.,Hildebrandt, P.,Hughes, J.
Structural insights into photoactivation and signalling in plant phytochromes.
Nat.Plants, 6:581-588, 2020

PubMed Abstract: Plant phytochromes are red/far-red photochromic photoreceptors that act as master regulators of development, controlling the expression of thousands of genes. Here, we describe the crystal structures of four plant phytochrome sensory modules, three at about 2 Å resolution or better, including the first of an A-type phytochrome. Together with extensive spectral data, these structures provide detailed insight into the structure and function of plant phytochromes. In the Pr state, the substitution of phycocyanobilin and phytochromobilin cofactors has no structural effect, nor does the amino-terminal extension play a significant functional role. Our data suggest that the chromophore propionates and especially the phytochrome-specific domain tongue act differently in plant and prokaryotic phytochromes. We find that the photoproduct in period-ARNT-single-minded (PAS)-cGMP-specific phosphodiesterase-adenylyl cyclase-FhlA (GAF) bidomains might represent a novel intermediate between MetaRc and Pfr. We also discuss the possible role of a likely nuclear localization signal specific to and conserved in the phytochrome A lineage.

PubMed: 32366982
DOI: 10.1038/s41477-020-0638-y

Experimental method

X-RAY DIFFRACTION (2.9 Å)