6TJA

Crystal structure of the SVS_A2 protein (W79F,G83L mutant) from ancestral sequence reconstruction at 2.27 A resolution

Summary for 6TJA

• Entry DOI: 10.2210/pdb6tja/pdb
• Descriptor: SVS_variant_AS1, DI(HYDROXYETHYL)ETHER (3 entities in total)
• Functional Keywords: terpene cyclase, engineered enzyme, biosynthetic protein
• Biological source: Streptomyces sp. CWA1
• Total number of polymer chains: 2
• Total formula weight: 81304.06

Authors

Rudraraju, R.,Schnell, R.,Schneider, G. (deposition date: 2019-11-25, release date: 2020-12-09, Last modification date: 2024-01-24)

• Primary citation: Schriever, K.,Saenz-Mendez, P.,Rudraraju, R.S.,Hendrikse, N.M.,Hudson, E.P.,Biundo, A.,Schnell, R.,Syren, P.O.; Engineering of Ancestors as a Tool to Elucidate Structure, Mechanism, and Specificity of Extant Terpene Cyclase.; J.Am.Chem.Soc., 143:3794-3807, 2021; PubMed: 33496585; DOI: 10.1021/jacs.0c10214

Experimental method

X-RAY DIFFRACTION (2.27 Å)