6TIL

Structure of A. niger Fdc WT in complex with FMN and 2 naphthoic acid

This is a non-PDB format compatible entry.

Summary for 6TIL

• Entry DOI: 10.2210/pdb6til/pdb
• Descriptor: Ferulic acid decarboxylase 1, MANGANESE (II) ION, POTASSIUM ION, ... (6 entities in total)
• Functional Keywords: (de)carboxylase, ubid, aromatic acid, prfmn, ligase
• Biological source: Aspergillus niger
• Total number of polymer chains: 1
• Total formula weight: 57097.60

Authors

Leys, D. (deposition date: 2019-11-22, release date: 2020-06-24, Last modification date: 2024-01-24)

Primary citation

Aleku, G.A.,Saaret, A.,Bradshaw-Allen, R.T.,Derrington, S.R.,Titchiner, G.R.,Gostimskaya, I.,Gahloth, D.,Parker, D.A.,Hay, S.,Leys, D.
Enzymatic C-H activation of aromatic compounds through CO 2 fixation.
Nat.Chem.Biol., 16:1255-1260, 2020

PubMed Abstract: The direct C-H carboxylation of aromatic compounds is an attractive route to the corresponding carboxylic acids, but remains challenging under mild conditions. It has been proposed that the first step in anaerobic microbial degradation of recalcitrant aromatic compounds is a UbiD-mediated carboxylation. In this study, we use the UbiD enzyme ferulic acid decarboxylase (Fdc) in combination with a carboxylic acid reductase to create aromatic degradation-inspired cascade reactions, leading to efficient functionalization of styrene through CO fixation. We reveal that rational structure-guided laboratory evolution can expand the substrate scope of Fdc, resulting in activity on a range of mono- and bicyclic aromatic compounds through a single mutation. Selected variants demonstrated 150-fold improvement in the conversion of coumarillic acid to benzofuran + CO and unlocked reactivity towards naphthoic acid. Our data demonstrate that UbiD-mediated C-H activation is a versatile tool for the transformation of aryl/alkene compounds and CO into commodity chemicals.

PubMed: 32719558
DOI: 10.1038/s41589-020-0603-0

Experimental method

X-RAY DIFFRACTION (1.42 Å)