6TIK

Hepatitis B virus core shell--virus-like particle with NadA epitope

6TIK の概要

• エントリーDOI: 10.2210/pdb6tik/pdb
• EMDBエントリー: 10316
• 分子名称: Capsid protein,Putative adhesin/invasin,Capsid protein,Factor H-binding protein (1 entity in total)
• 機能のキーワード: virus-like particle, vlp, antigen, nada, neisseria meningitidis, hbv, hbc, factor h binding protein, virus like particle
• 由来する生物種: Hepatitis B virus (HBV); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 248467.00

構造登録者

Roseman, A.M.,Colllins, R.F.,Derrick, J.P. (登録日: 2019-11-22, 公開日: 2020-04-29, 最終更新日: 2024-11-13)

主引用文献

Aston-Deaville, S.,Carlsson, E.,Saleem, M.,Thistlethwaite, A.,Chan, H.,Maharjan, S.,Facchetti, A.,Feavers, I.M.,Alistair Siebert, C.,Collins, R.F.,Roseman, A.,Derrick, J.P.
An assessment of the use of Hepatitis B Virus core protein virus-like particles to display heterologous antigens from Neisseria meningitidis.
Vaccine, 38:3201-3209, 2020

PubMed Abstract: Neisseria meningitidis is the causative agent of meningococcal meningitis and sepsis and remains a significant public health problem in many countries. Efforts to develop a comprehensive vaccine against serogroup B meningococci have focused on the use of surface-exposed outer membrane proteins. Here we report the use of virus-like particles derived from the core protein of Hepatitis B Virus, HBc, to incorporate antigen domains derived from Factor H binding protein (FHbp) and the adhesin NadA. The extracellular domain of NadA was inserted into the major immunodominant region of HBc, and the C-terminal domain of FHbp at the C-terminus (CFHbp), creating a single polypeptide chain 3.7-fold larger than native HBc. Remarkably, cryoelectron microscopy revealed that the construct formed assemblies that were able to incorporate both antigens with minimal structural changes to native HBc. Electron density was weak for NadA and absent for CFHbp, partly attributable to domain flexibility. Following immunization of mice, three HBc fusions (CFHbp or NadA alone, NadA + CFHbp) were able to induce production of IgG1, IgG2a and IgG2b antibodies reactive against their respective antigens at dilutions in excess of 1:18,000. However, only HBc fusions containing NadA elicited the production of antibodies with serum bactericidal activity. It is hypothesized that this improved immune response is attributable to the adoption of a more native-like folding of crucial conformational epitopes of NadA within the chimeric VLP. This work demonstrates that HBc can incorporate insertions of large antigen domains but that maintenance of their three-dimensional structure is likely to be critical in obtaining a protective response.

PubMed: 32178907
DOI: 10.1016/j.vaccine.2020.03.001

実験手法

ELECTRON MICROSCOPY (3.4 Å)