6TIJ の概要
| エントリーDOI | 10.2210/pdb6tij/pdb |
| 分子名称 | Ferulic acid decarboxylase 1, MANGANESE (II) ION, POTASSIUM ION, ... (6 entities in total) |
| 機能のキーワード | (de)carboxylase, ubid, aromatic acid, prfmn, ligase |
| 由来する生物種 | Aspergillus niger |
| タンパク質・核酸の鎖数 | 1 |
| 化学式量合計 | 57333.87 |
| 構造登録者 | |
| 主引用文献 | Aleku, G.A.,Saaret, A.,Bradshaw-Allen, R.T.,Derrington, S.R.,Titchiner, G.R.,Gostimskaya, I.,Gahloth, D.,Parker, D.A.,Hay, S.,Leys, D. Enzymatic C-H activation of aromatic compounds through CO 2 fixation. Nat.Chem.Biol., 16:1255-1260, 2020 Cited by PubMed Abstract: The direct C-H carboxylation of aromatic compounds is an attractive route to the corresponding carboxylic acids, but remains challenging under mild conditions. It has been proposed that the first step in anaerobic microbial degradation of recalcitrant aromatic compounds is a UbiD-mediated carboxylation. In this study, we use the UbiD enzyme ferulic acid decarboxylase (Fdc) in combination with a carboxylic acid reductase to create aromatic degradation-inspired cascade reactions, leading to efficient functionalization of styrene through CO fixation. We reveal that rational structure-guided laboratory evolution can expand the substrate scope of Fdc, resulting in activity on a range of mono- and bicyclic aromatic compounds through a single mutation. Selected variants demonstrated 150-fold improvement in the conversion of coumarillic acid to benzofuran + CO and unlocked reactivity towards naphthoic acid. Our data demonstrate that UbiD-mediated C-H activation is a versatile tool for the transformation of aryl/alkene compounds and CO into commodity chemicals. PubMed: 32719558DOI: 10.1038/s41589-020-0603-0 主引用文献が同じPDBエントリー |
| 実験手法 | X-RAY DIFFRACTION (1.12 Å) |
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