6TH8

Reconstructing the Origins of the HemD-like fold

6TH8 の概要

• エントリーDOI: 10.2210/pdb6th8/pdb
• NMR情報: BMRB: 34452
• 分子名称: cU3Sd (1 entity in total)
• 機能のキーワード: protein design, hemd-like fold, flavodoxin-like fold, flavoprotein
• 由来する生物種: Thermus thermophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13651.65

構造登録者

Coles, M.,Toledo-Patino, S.,Chaubey, M.,Hocker, B. (登録日: 2019-11-19, 公開日: 2019-11-27, 最終更新日: 2024-06-19)

主引用文献

Toledo-Patino, S.,Chaubey, M.,Coles, M.,Hocker, B.
Reconstructing the Remote Origins of a Fold Singleton from a Flavodoxin-Like Ancestor.
Biochemistry, 58:4790-4793, 2019

PubMed Abstract: Evolutionary processes that led to the emergence of structured protein domains left footprints in the sequences of modern proteins. We searched for such hints employing state-of-the-art sequence analysis and found evidence that the HemD-like fold emerged from the flavodoxin-like fold through segment swap and gene duplication. To verify this hypothesis, we reverted these evolutionary steps experimentally, constructing a HemD-half that resulted in a protein with the canonical flavodoxin-like architecture. These results of fold reconstruction from the sequence of a different fold strongly support our hypothesis of common ancestry. It further illustrates the plasticity of modern proteins to form new folded proteins.

PubMed: 31724394
DOI: 10.1021/acs.biochem.9b00900

実験手法

SOLUTION NMR