6TH0

Crystal structure of Arabidopsis thaliana NAA60 in complex with acetyl-CoA

6TH0 の概要

• エントリーDOI: 10.2210/pdb6th0/pdb
• 関連するPDBエントリー: 6tgx
• 分子名称: Acyl-CoA N-acyltransferases (NAT) superfamily protein, ACETYL COENZYME *A (3 entities in total)
• 機能のキーワード: n-alpha-acetyltransferase naa60 acetyl-coa natf, plant protein
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43188.88

構造登録者

Layer, D.,Kopp, J.,Lapouge, K.,Sinning, I. (登録日: 2019-11-18, 公開日: 2020-06-24, 最終更新日: 2024-01-24)

主引用文献

Linster, E.,Layer, D.,Bienvenut, W.V.,Dinh, T.V.,Weyer, F.A.,Leemhuis, W.,Brunje, A.,Hoffrichter, M.,Miklankova, P.,Kopp, J.,Lapouge, K.,Sindlinger, J.,Schwarzer, D.,Meinnel, T.,Finkemeier, I.,Giglione, C.,Hell, R.,Sinning, I.,Wirtz, M.
The Arabidopsis N alpha -acetyltransferase NAA60 locates to the plasma membrane and is vital for the high salt stress response.
New Phytol., 228:554-569, 2020

PubMed Abstract: In humans and plants, N-terminal acetylation plays a central role in protein homeostasis, affects 80% of proteins in the cytoplasm and is catalyzed by five ribosome-associated N-acetyltransferases (NatA-E). Humans also possess a Golgi-associated NatF (HsNAA60) that is essential for Golgi integrity. Remarkably, NAA60 is absent in fungi and has not been identified in plants. Here we identify and characterize the first plasma membrane-anchored post-translationally acting N-acetyltransferase AtNAA60 in the reference plant Arabidopsis thaliana by the combined application of reverse genetics, global proteomics, live-cell imaging, microscale thermophoresis, circular dichroism spectroscopy, nano-differential scanning fluorometry, intrinsic tryptophan fluorescence and X-ray crystallography. We demonstrate that AtNAA60, like HsNAA60, is membrane-localized in vivo by an α-helical membrane anchor at its C-terminus, but in contrast to HsNAA60, AtNAA60 localizes to the plasma membrane. The AtNAA60 crystal structure provides insights into substrate-binding, the broad substrate specificity and the catalytic mechanism probed by structure-based mutagenesis. Characterization of the NAA60 loss-of-function mutants (naa60-1 and naa60-2) uncovers a plasma membrane-localized substrate of AtNAA60 and the importance of NAA60 during high salt stress. Our findings provide evidence for the plant-specific evolution of a plasma membrane-anchored N-acetyltransferase that is vital for adaptation to stress.

PubMed: 32548857
DOI: 10.1111/nph.16747

実験手法

X-RAY DIFFRACTION (1.75 Å)