6TFS

Structure in P3212 form of the PBP/SBP MoaA in complex with glucopinic acid from A.tumefacien R10

6TFS の概要

• エントリーDOI: 10.2210/pdb6tfs/pdb
• 関連するPDBエントリー: 6TFQ
• 分子名称: ABC transporter substrate-binding protein, (2~{S})-2-[[(3~{S},4~{R},5~{R})-3,4,5,6-tetrakis(oxidanyl)-2-oxidanylidene-hexyl]amino]pentanedioic acid, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: solute binding protein, periplasmic binding protein, transport protein
• 由来する生物種: Rhizobium radiobacter (Agrobacterium tumefaciens)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 113360.51

構造登録者

Morera, S.,Vigouroux, A. (登録日: 2019-11-14, 公開日: 2020-01-22, 最終更新日: 2024-01-24)

主引用文献

Vigouroux, A.,Dore, J.,Marty, L.,Aumont-Nicaise, M.,Legrand, P.,Dessaux, Y.,Vial, L.,Morera, S.
Import pathways of the mannityl-opines into the bacterial pathogen Agrobacterium tumefaciens: structural, affinity and in vivo approaches.
Biochem.J., 477:615-628, 2020

PubMed Abstract: Agrobacterium tumefaciens pathogens use specific compounds denoted opines as nutrients in their plant tumor niche. These opines are produced by the host plant cells genetically modified by agrobacteria. They are imported into bacteria via solute-binding proteins (SBPs) in association with ATP-binding cassette transporters. The mannityl-opine family encompasses mannopine, mannopinic acid, agropine and agropinic acid. Structural and affinity data on mannopinic acid bound to SBPs are currently lacking while those of the three others mannityl opines are available. We investigated the molecular basis of two pathways for mannopinic acid uptake. MoaA was proposed as the specific SBP for mannopinic acid import in mannityl opines-assimilating agrobacteria, which was validated here using genetic studies and affinity measurements. We structurally characterized the mannopinic acid-binding mode of MoaA in two crystal forms at 2.05 and 1.57 Å resolution. We demonstrated that the non-specific SBP MotA, so far characterized as mannopine and Amadori compound importer, was also able to transport mannopinic acid. The structure of MotA bound to mannopinic acid at 2.2 Å resolution defines a different mannopinic acid-binding signature, similar to that of mannopine. Combining in vitro and in vivo approaches, this work allowed us to complete the characterization of the mannityl-opines assimilation pathways, highlighting the important role of two dual imports of agropinic and mannopinic acids. Our data shed new light on how the mannityl-opines contribute to the establishment of the ecological niche of agrobacteria from the early to the late stages of tumor development.

PubMed: 31922182
DOI: 10.1042/BCJ20190886

実験手法

X-RAY DIFFRACTION (2 Å)