6TE8

Neck of native GTA particle computed with C12 symmetry

6TE8 の概要

• エントリーDOI: 10.2210/pdb6te8/pdb
• 関連するPDBエントリー: 6TBA
• EMDBエントリー: 10443 10476 10477 10541 10542
• 分子名称: Adaptor protein Rcc01688, Phage portal protein, HK97 family (2 entities in total)
• 機能のキーワード: "portal", "gene transfer agent", "bacteriophage", "adaptor", virus
• 由来する生物種: Rhodobacter capsulatus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 63546.02

構造登録者

Bardy, P.,Fuzik, T.,Hrebik, D.,Pantucek, R.,Beatty, J.T.,Plevka, P. (登録日: 2019-11-11, 公開日: 2020-07-22, 最終更新日: 2024-05-22)

主引用文献

Bardy, P.,Fuzik, T.,Hrebik, D.,Pantucek, R.,Thomas Beatty, J.,Plevka, P.
Structure and mechanism of DNA delivery of a gene transfer agent.
Nat Commun, 11:3034-3034, 2020

PubMed Abstract: Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm.

PubMed: 32541663
DOI: 10.1038/s41467-020-16669-9

実験手法

ELECTRON MICROSCOPY (3.32 Å)