6TDZ

Cryo-EM structure of Euglena gracilis mitochondrial ATP synthase, OSCP/F1/c-ring, rotational state 2

6TDZ の概要

• エントリーDOI: 10.2210/pdb6tdz/pdb
• EMDBエントリー: 10472
• 分子名称: oligomycin sensitivity conferring protein (OSCP), p18, inhibitor of F1 (IF1), ... (15 entities in total)
• 機能のキーワード: mitochondria, atp synthase, membrane protein
• 由来する生物種: Euglena gracilis; 詳細
• タンパク質・核酸の鎖数: 26
• 化学式量合計: 696890.29

構造登録者

Muhleip, A.,Amunts, A. (登録日: 2019-11-10, 公開日: 2019-11-27, 最終更新日: 2024-05-22)

主引用文献

Muhleip, A.,McComas, S.E.,Amunts, A.
Structure of a mitochondrial ATP synthase with bound native cardiolipin.
Elife, 8:-, 2019

PubMed Abstract: The mitochondrial ATP synthase fuels eukaryotic cells with chemical energy. Here we report the cryo-EM structure of a divergent ATP synthase dimer from mitochondria of , a member of the phylum Euglenozoa that also includes human parasites. It features 29 different subunits, 8 of which are newly identified. The membrane region was determined to 2.8 Å resolution, enabling the identification of 37 associated lipids, including 25 cardiolipins, which provides insight into protein-lipid interactions and their functional roles. The rotor-stator interface comprises four membrane-embedded horizontal helices, including a distinct subunit . The dimer interface is formed entirely by phylum-specific components, and a peripherally associated subcomplex contributes to the membrane curvature. The central and peripheral stalks directly interact with each other. Last, the ATPase inhibitory factor 1 (IF) binds in a mode that is different from human, but conserved in Trypanosomatids.

PubMed: 31738165
DOI: 10.7554/eLife.51179

実験手法

ELECTRON MICROSCOPY (3.14 Å)