6TDN

Bam_5925cDD 5924nDD docking domains

6TDN の概要

• エントリーDOI: 10.2210/pdb6tdn/pdb
• NMR情報: BMRB: 34448
• 分子名称: Beta-ketoacyl synthase,Beta-ketoacyl synthase (1 entity in total)
• 機能のキーワード: docking domain, polyketide synthase, protein binding
• 由来する生物種: Burkholderia ambifaria AMMD; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 18300.18

構造登録者

Risser, F.,Chagot, B. (登録日: 2019-11-08, 公開日: 2020-08-12, 最終更新日: 2024-06-19)

主引用文献

Risser, F.,Collin, S.,Dos Santos-Morais, R.,Gruez, A.,Chagot, B.,Weissman, K.J.
Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: Docking in the enacyloxin IIa polyketide synthase.
J.Struct.Biol., 212:107581-107581, 2020

PubMed Abstract: Modular polyketide synthases (PKSs) are molecular-scale assembly lines comprising multiple gigantic polypeptide subunits. Faithful ordering of the subunits is mediated by extreme C- and N-terminal regions called docking domains (DDs). Decrypting how specificity is achieved by these elements is important both for understanding PKS function and modifying it to generate useful polyketide analogues for biological evaluation. Here we report the biophysical and structural characterisation of all six PKS/PKS interfaces in the unusual, chimaeric cis-AT/trans-AT PKS pathway responsible for biosynthesis of the antibiotic enacyloxin IIa in Burkholderia ambifaria. Taken together with previous work, our data reveal that specificity is achieved in the enacyloxin PKS by deploying at least three functionally orthogonal classes of DDs. We also demonstrate for the first time that cis-AT PKS subunits incorporate DDs with intrinsically disordered character, reinforcing the utility of such regions for achieving both medium affinity and high specificity at PKS intersubunit junctions. Overall, this work substantially increases the number of orthogonal DDs available for creating novel, highly-specific interfaces within cis- and trans-AT PKSs and their hybrids. It also reveals unexpected sequence/structure relationships in PKS DDs, identifying major current limitations to both accurately predicting and categorising these useful protein-protein interaction motifs.

PubMed: 32717326
DOI: 10.1016/j.jsb.2020.107581

実験手法

SOLUTION NMR