6TC7

PAS-GAF bidomain of Glycine max phytochromeA

これはPDB形式変換不可エントリーです。

6TC7 の概要

• エントリーDOI: 10.2210/pdb6tc7/pdb
• 分子名称: Phytochrome, PHYCOCYANOBILIN, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: phytochrome, plant protein
• 由来する生物種: Glycine max (Soybean)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 81442.28

構造登録者

Nagano, S.,Guan, K.,Shenkutie, S.M.,Hughes, J.E. (登録日: 2019-11-05, 公開日: 2020-05-06, 最終更新日: 2024-11-06)

主引用文献

Nagano, S.,Guan, K.,Shenkutie, S.M.,Feiler, C.,Weiss, M.,Kraskov, A.,Buhrke, D.,Hildebrandt, P.,Hughes, J.
Structural insights into photoactivation and signalling in plant phytochromes.
Nat.Plants, 6:581-588, 2020

PubMed Abstract: Plant phytochromes are red/far-red photochromic photoreceptors that act as master regulators of development, controlling the expression of thousands of genes. Here, we describe the crystal structures of four plant phytochrome sensory modules, three at about 2 Å resolution or better, including the first of an A-type phytochrome. Together with extensive spectral data, these structures provide detailed insight into the structure and function of plant phytochromes. In the Pr state, the substitution of phycocyanobilin and phytochromobilin cofactors has no structural effect, nor does the amino-terminal extension play a significant functional role. Our data suggest that the chromophore propionates and especially the phytochrome-specific domain tongue act differently in plant and prokaryotic phytochromes. We find that the photoproduct in period-ARNT-single-minded (PAS)-cGMP-specific phosphodiesterase-adenylyl cyclase-FhlA (GAF) bidomains might represent a novel intermediate between MetaRc and Pfr. We also discuss the possible role of a likely nuclear localization signal specific to and conserved in the phytochrome A lineage.

PubMed: 32366982
DOI: 10.1038/s41477-020-0638-y

実験手法

X-RAY DIFFRACTION (2.13 Å)