6TBA

Virion of native gene transfer agent (GTA) particle

これはPDB形式変換不可エントリーです。

6TBA の概要

• エントリーDOI: 10.2210/pdb6tba/pdb
• 関連するPDBエントリー: 6TUI
• EMDBエントリー: 10442 10443 10476 10477 10478 10479 10490 10592
• 分子名称: Phage major capsid protein, HK97 family, Uncharacterized protein, IRON/SULFUR CLUSTER, ... (12 entities in total)
• 機能のキーワード: "virion", "horizontal gene transfer", "gene delivery", "hk97", virus
• 由来する生物種: Rhodobacter capsulatus SB 1003; 詳細
• タンパク質・核酸の鎖数: 288
• 化学式量合計: 8765993.43

構造登録者

Bardy, P.,Fuzik, T.,Hrebik, D.,Pantucek, R.,Beatty, J.T.,Plevka, P. (登録日: 2019-11-01, 公開日: 2020-07-22, 最終更新日: 2024-05-22)

主引用文献

Bardy, P.,Fuzik, T.,Hrebik, D.,Pantucek, R.,Thomas Beatty, J.,Plevka, P.
Structure and mechanism of DNA delivery of a gene transfer agent.
Nat Commun, 11:3034-3034, 2020

PubMed Abstract: Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral gene exchange. However, the mechanism by which GTAs deliver DNA into cells is unknown. Here we present the structure of the GTA of Rhodobacter capsulatus (RcGTA) and describe the conformational changes required for its DNA ejection. The structure of RcGTA resembles that of a tailed phage, but it has an oblate head shortened in the direction of the tail axis, which limits its packaging capacity to less than 4,500 base pairs of linear double-stranded DNA. The tail channel of RcGTA contains a trimer of proteins that possess features of both tape measure proteins of long-tailed phages from the family Siphoviridae and tail needle proteins of short-tailed phages from the family Podoviridae. The opening of a constriction within the RcGTA baseplate enables the ejection of DNA into bacterial periplasm.

PubMed: 32541663
DOI: 10.1038/s41467-020-16669-9

実験手法

ELECTRON MICROSCOPY (4.54 Å)