6TB3

yeast 80S ribosome in complex with the Not5 subunit of the CCR4-NOT complex

これはPDB形式変換不可エントリーです。

6TB3 の概要

• エントリーDOI: 10.2210/pdb6tb3/pdb
• EMDBエントリー: 10431
• 分子名称: Saccharomyces cerevisiae S288C 18S ribosomal RNA (RDN18-1), rRNA, 40S ribosomal protein S6-A, 40S ribosomal protein S7-A, ... (86 entities in total)
• 機能のキーワード: not5, ccr4-not complex, translation control, translation
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 83
• 化学式量合計: 3041158.64

構造登録者

Buschauer, R.,Cheng, J.,Berninghausen, O.,Tesina, P.,Becker, T.,Beckmann, R. (登録日: 2019-10-31, 公開日: 2020-04-22, 最終更新日: 2024-05-22)

主引用文献

Buschauer, R.,Matsuo, Y.,Sugiyama, T.,Chen, Y.H.,Alhusaini, N.,Sweet, T.,Ikeuchi, K.,Cheng, J.,Matsuki, Y.,Nobuta, R.,Gilmozzi, A.,Berninghausen, O.,Tesina, P.,Becker, T.,Coller, J.,Inada, T.,Beckmann, R.
The Ccr4-Not complex monitors the translating ribosome for codon optimality.
Science, 368:-, 2020

PubMed Abstract: Control of messenger RNA (mRNA) decay rate is intimately connected to translation elongation, but the spatial coordination of these events is poorly understood. The Ccr4-Not complex initiates mRNA decay through deadenylation and activation of decapping. We used a combination of cryo-electron microscopy, ribosome profiling, and mRNA stability assays to examine the recruitment of Ccr4-Not to the ribosome via specific interaction of the Not5 subunit with the ribosomal E-site in This interaction occurred when the ribosome lacked accommodated A-site transfer RNA, indicative of low codon optimality. Loss of the interaction resulted in the inability of the mRNA degradation machinery to sense codon optimality. Our findings elucidate a physical link between the Ccr4-Not complex and the ribosome and provide mechanistic insight into the coupling of decoding efficiency with mRNA stability.

PubMed: 32299921
DOI: 10.1126/science.aay6912

実験手法

ELECTRON MICROSCOPY (2.8 Å)