6T93

Nucleosome with OCT4-SOX2 motif at SHL-6

6T93 の概要

• エントリーDOI: 10.2210/pdb6t93/pdb
• EMDBエントリー: 10408
• 分子名称: Histone H3.1, Histone H4, Histone H2A type 1-B/E, ... (6 entities in total)
• 機能のキーワード: nucleosome, oct4, sox2, transcription factor, transcription
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 206572.25

構造登録者

Michael, A.K.,Kempf, G.,Cavadini, S.,Bunker, R.D.,Thoma, N.H. (登録日: 2019-10-25, 公開日: 2020-05-06, 最終更新日: 2024-05-22)

主引用文献

Michael, A.K.,Grand, R.S.,Isbel, L.,Cavadini, S.,Kozicka, Z.,Kempf, G.,Bunker, R.D.,Schenk, A.D.,Graff-Meyer, A.,Pathare, G.R.,Weiss, J.,Matsumoto, S.,Burger, L.,Schubeler, D.,Thoma, N.H.
Mechanisms of OCT4-SOX2 motif readout on nucleosomes.
Science, 368:1460-1465, 2020

PubMed Abstract: Transcription factors (TFs) regulate gene expression through chromatin where nucleosomes restrict DNA access. To study how TFs bind nucleosome-occupied motifs, we focused on the reprogramming factors OCT4 and SOX2 in mouse embryonic stem cells. We determined TF engagement throughout a nucleosome at base-pair resolution in vitro, enabling structure determination by cryo-electron microscopy at two preferred positions. Depending on motif location, OCT4 and SOX2 differentially distort nucleosomal DNA. At one position, OCT4-SOX2 removes DNA from histone H2A and histone H3; however, at an inverted motif, the TFs only induce local DNA distortions. OCT4 uses one of its two DNA-binding domains to engage DNA in both structures, reading out a partial motif. These findings explain site-specific nucleosome engagement by the pluripotency factors OCT4 and SOX2, and they reveal how TFs distort nucleosomes to access chromatinized motifs.

PubMed: 32327602
DOI: 10.1126/science.abb0074

実験手法

ELECTRON MICROSCOPY (3.49 Å)