6T8D

The cytotoxin MakB from Vibrio cholerae

6T8D の概要

• エントリーDOI: 10.2210/pdb6t8d/pdb
• 分子名称: MakB, SULFATE ION (3 entities in total)
• 機能のキーワード: cytotoxin, virulence factor, toxin
• 由来する生物種: Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42121.43

構造登録者

Persson, K.,Nagampalli, R. (登録日: 2019-10-24, 公開日: 2020-11-18, 最終更新日: 2024-10-16)

主引用文献

Nadeem, A.,Nagampalli, R.,Toh, E.,Alam, A.,Myint, S.L.,Heidler, T.V.,Dongre, M.,Zlatkov, N.,Pace, H.,Bano, F.,Sjostedt, A.,Bally, M.,Uhlin, B.E.,Wai, S.N.,Persson, K.
A tripartite cytolytic toxin formed by Vibrio cholerae proteins with flagellum-facilitated secretion.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: The protein MakA was discovered as a motility-associated secreted toxin from Here, we show that MakA is part of a gene cluster encoding four additional proteins: MakB, MakC, MakD, and MakE. MakA, MakB, and MakE were readily detected in culture supernatants of wild-type , whereas secretion was very much reduced from a flagellum-deficient mutant. Crystal structures of MakA, MakB, and MakE revealed a structural relationship to a superfamily of bacterial pore-forming toxins. Expression of MakA/B/E in resulted in toxicity toward used as a predatory model organism. None of these Mak proteins alone or in pairwise combinations were cytolytic, but an equimolar mixture of MakA, MakB, and MakE acted as a tripartite cytolytic toxin in vitro, causing lysis of erythrocytes and cytotoxicity on cultured human colon carcinoma cells. Formation of oligomeric complexes on liposomes was observed by electron microscopy. Oligomer interaction with membranes was initiated by MakA membrane binding followed by MakB and MakE joining the assembly of a pore structure. A predicted membrane insertion domain of MakA was shown by site-directed mutagenesis to be essential for toxicity toward Bioinformatic analyses revealed that the gene cluster is present as a genomic island in the vast majority of sequenced genomes of and the fish pathogen We suggest that the hitherto-unrecognized cytolytic MakA/B/E toxin can contribute to Vibrionaceae fitness and virulence potential in different host environments and organisms.

PubMed: 34799450
DOI: 10.1073/pnas.2111418118

実験手法

X-RAY DIFFRACTION (2.1 Å)