6T7I

Structure of yeast 80S ribosome stalled on the CGA-CGA inhibitory codon combination.

これはPDB形式変換不可エントリーです。

6T7I の概要

• エントリーDOI: 10.2210/pdb6t7i/pdb
• EMDBエントリー: 10396
• 分子名称: 18S ribosomal RNA, 40S ribosomal protein S7-A, 40S ribosomal protein S8-A, ... (81 entities in total)
• 機能のキーワード: stalling dicodon codon pair, translation
• 由来する生物種: Saccharomyces cerevisiae; 詳細
• タンパク質・核酸の鎖数: 81
• 化学式量合計: 3160037.04

構造登録者

Tesina, P.,Buschauer, R.,Cheng, J.,Berninghausen, O.,Becker, R.,Beckmann, R. (登録日: 2019-10-22, 公開日: 2019-12-25, 最終更新日: 2024-05-22)

主引用文献

Tesina, P.,Lessen, L.N.,Buschauer, R.,Cheng, J.,Wu, C.C.,Berninghausen, O.,Buskirk, A.R.,Becker, T.,Beckmann, R.,Green, R.
Molecular mechanism of translational stalling by inhibitory codon combinations and poly(A) tracts.
Embo J., 39:e103365-e103365, 2020

PubMed Abstract: Inhibitory codon pairs and poly(A) tracts within the translated mRNA cause ribosome stalling and reduce protein output. The molecular mechanisms that drive these stalling events, however, are still unknown. Here, we use a combination of in vitro biochemistry, ribosome profiling, and cryo-EM to define molecular mechanisms that lead to these ribosome stalls. First, we use an in vitro reconstituted yeast translation system to demonstrate that inhibitory codon pairs slow elongation rates which are partially rescued by increased tRNA concentration or by an artificial tRNA not dependent on wobble base-pairing. Ribosome profiling data extend these observations by revealing that paused ribosomes with empty A sites are enriched on these sequences. Cryo-EM structures of stalled ribosomes provide a structural explanation for the observed effects by showing decoding-incompatible conformations of mRNA in the A sites of all studied stall- and collision-inducing sequences. Interestingly, in the case of poly(A) tracts, the inhibitory conformation of the mRNA in the A site involves a nucleotide stacking array. Together, these data demonstrate a novel mRNA-induced mechanisms of translational stalling in eukaryotic ribosomes.

PubMed: 31858614
DOI: 10.15252/embj.2019103365

実験手法

ELECTRON MICROSCOPY (3.2 Å)