6T73

New antiparallel dimer of aureochrome 1a LOV domain mutants from Phaeodactylum tricornutum

Summary for 6T73

• Entry DOI: 10.2210/pdb6t73/pdb
• Descriptor: Ptaureo1a lov2 domain, FLAVIN MONONUCLEOTIDE, CHLORIDE ION (3 entities in total)
• Functional Keywords: photoreceptor, interface, flavoprotein
• Biological source: Phaeodactylum tricornutum (Diatom)
• Total number of polymer chains: 3
• Total formula weight: 55547.01

Authors

Essen, L.O.,Hepp, S. (deposition date: 2019-10-21, release date: 2020-03-18, Last modification date: 2024-01-24)

Primary citation

Hepp, S.,Trauth, J.,Hasenjager, S.,Bezold, F.,Essen, L.O.,Taxis, C.
An Optogenetic Tool for Induced Protein Stabilization Based on the Phaeodactylum tricornutum Aureochrome 1a Light-Oxygen-Voltage Domain.
J.Mol.Biol., 432:1880-1900, 2020

PubMed Abstract: Control of cellular events by optogenetic tools is a powerful approach to manipulate cellular functions in a minimally invasive manner. A common problem posed by the application of optogenetic tools is to tune the activity range to be physiologically relevant. Here, we characterized a photoreceptor of the light-oxygen-voltage (LOV) domain family of Phaeodactylum tricornutum aureochrome 1a (AuLOV) as a tool for increasing protein stability under blue light conditions in budding yeast. Structural studies of AuLOV, the variants AuLOV, and AuLOV revealed alternative dimer association modes for the dark state, which differ from previously reported AuLOV dark-state structures. Rational design of AuLOV-dimer interface mutations resulted in an optimized optogenetic tool that we fused to the photoactivatable adenylyl cyclase from Beggiatoa sp. This synergistic light-regulation approach using two photoreceptors resulted in an optimized, photoactivatable adenylyl cyclase with a cyclic adenosine monophosphate production activity that matches the physiological range of Saccharomyces cerevisiae. Overall, we enlarged the optogenetic toolbox for yeast and demonstrated the importance of fine-tuning the optogenetic tool activity for successful application in cells.

PubMed: 32105734
DOI: 10.1016/j.jmb.2020.02.019

Experimental method

X-RAY DIFFRACTION (3.435 Å)