6T73

New antiparallel dimer of aureochrome 1a LOV domain mutants from Phaeodactylum tricornutum

6T73 の概要

• エントリーDOI: 10.2210/pdb6t73/pdb
• 分子名称: Ptaureo1a lov2 domain, FLAVIN MONONUCLEOTIDE, CHLORIDE ION (3 entities in total)
• 機能のキーワード: photoreceptor, interface, flavoprotein
• 由来する生物種: Phaeodactylum tricornutum (Diatom)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 55547.01

構造登録者

Essen, L.O.,Hepp, S. (登録日: 2019-10-21, 公開日: 2020-03-18, 最終更新日: 2024-01-24)

主引用文献

Hepp, S.,Trauth, J.,Hasenjager, S.,Bezold, F.,Essen, L.O.,Taxis, C.
An Optogenetic Tool for Induced Protein Stabilization Based on the Phaeodactylum tricornutum Aureochrome 1a Light-Oxygen-Voltage Domain.
J.Mol.Biol., 432:1880-1900, 2020

PubMed Abstract: Control of cellular events by optogenetic tools is a powerful approach to manipulate cellular functions in a minimally invasive manner. A common problem posed by the application of optogenetic tools is to tune the activity range to be physiologically relevant. Here, we characterized a photoreceptor of the light-oxygen-voltage (LOV) domain family of Phaeodactylum tricornutum aureochrome 1a (AuLOV) as a tool for increasing protein stability under blue light conditions in budding yeast. Structural studies of AuLOV, the variants AuLOV, and AuLOV revealed alternative dimer association modes for the dark state, which differ from previously reported AuLOV dark-state structures. Rational design of AuLOV-dimer interface mutations resulted in an optimized optogenetic tool that we fused to the photoactivatable adenylyl cyclase from Beggiatoa sp. This synergistic light-regulation approach using two photoreceptors resulted in an optimized, photoactivatable adenylyl cyclase with a cyclic adenosine monophosphate production activity that matches the physiological range of Saccharomyces cerevisiae. Overall, we enlarged the optogenetic toolbox for yeast and demonstrated the importance of fine-tuning the optogenetic tool activity for successful application in cells.

PubMed: 32105734
DOI: 10.1016/j.jmb.2020.02.019

実験手法

X-RAY DIFFRACTION (3.435 Å)