6T51
NMR structure of KRAS22RT G-quadruplex forming within KRAS promoter region at physological temperature
Summary for 6T51
| Entry DOI | 10.2210/pdb6t51/pdb |
| NMR Information | BMRB: 27173 |
| Descriptor | KRAS22RT, POTASSIUM ION (2 entities in total) |
| Functional Keywords | kras oncogene, promoter region, g-quadruplex, dna |
| Biological source | Homo sapiens |
| Total number of polymer chains | 1 |
| Total formula weight | 7064.71 |
| Authors | Marquevielle, J.,Salgado, G. (deposition date: 2019-10-15, release date: 2019-10-30, Last modification date: 2024-05-15) |
| Primary citation | Marquevielle, J.,Kumar, M.V.V.,Mergny, J.L.,Salgado, G.F. 1H,13C, and15N chemical shift assignments of a G-quadruplex forming sequence within the KRAS proto-oncogene promoter region. Biomol.Nmr Assign., 12:123-127, 2018 Cited by PubMed Abstract: Single stranded guanine rich DNA (or RNA) sequences adopt noncanonical secondary structures called G-quadruplexes (G4). Functionally, quadruplexes control gene transcription and regulate activities such as replication, gene recombination or alternative splicing. Hence they are potential targets for cancer, neuronal, and viral related diseases. KRAS is one of the most mutated oncogenes in the genome of cancer cells and contains a nuclease hypersensitive element (NHE) sequence capable of forming G-quadruplexes via its six runs of guanines. In our work, we are interested in the NMR structure of the major G4 scaffold formed in the KRAS NHE region with a mutated sequence of 22 residues. Here, we report H, C and N chemical shift assignments the G4 formed within KRAS22RT sequence. PubMed: 29189986DOI: 10.1007/s12104-017-9793-0 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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