6T51

NMR structure of KRAS22RT G-quadruplex forming within KRAS promoter region at physological temperature

6T51 の概要

• エントリーDOI: 10.2210/pdb6t51/pdb
• NMR情報: BMRB: 27173
• 分子名称: KRAS22RT, POTASSIUM ION (2 entities in total)
• 機能のキーワード: kras oncogene, promoter region, g-quadruplex, dna
• 由来する生物種: Homo sapiens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7064.71

構造登録者

Marquevielle, J.,Salgado, G. (登録日: 2019-10-15, 公開日: 2019-10-30, 最終更新日: 2024-05-15)

主引用文献

Marquevielle, J.,Kumar, M.V.V.,Mergny, J.L.,Salgado, G.F.
1H,13C, and15N chemical shift assignments of a G-quadruplex forming sequence within the KRAS proto-oncogene promoter region.
Biomol.Nmr Assign., 12:123-127, 2018

PubMed Abstract: Single stranded guanine rich DNA (or RNA) sequences adopt noncanonical secondary structures called G-quadruplexes (G4). Functionally, quadruplexes control gene transcription and regulate activities such as replication, gene recombination or alternative splicing. Hence they are potential targets for cancer, neuronal, and viral related diseases. KRAS is one of the most mutated oncogenes in the genome of cancer cells and contains a nuclease hypersensitive element (NHE) sequence capable of forming G-quadruplexes via its six runs of guanines. In our work, we are interested in the NMR structure of the major G4 scaffold formed in the KRAS NHE region with a mutated sequence of 22 residues. Here, we report H, C and N chemical shift assignments the G4 formed within KRAS22RT sequence.

PubMed: 29189986
DOI: 10.1007/s12104-017-9793-0

実験手法

SOLUTION NMR