6T3O

Crystal structure of the human myomesin domain 10

6T3O の概要

• エントリーDOI: 10.2210/pdb6t3o/pdb
• 分子名称: Myomesin-1, AZIDE ION (3 entities in total)
• 機能のキーワード: small protein domain, scaffold structure, contractile protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15437.46

構造登録者

Duskova, J.,Petrokova, H.,Maly, P. (登録日: 2019-10-11, 公開日: 2021-03-17, 最終更新日: 2024-01-24)

主引用文献

Kuchar, M.,Kosztyu, P.,Daniel Liskova, V.,Cerny, J.,Petrokova, H.,Vroblova, E.,Maly, M.,Vankova, L.,Krupka, M.,Raskova Kafkova, L.,Turanek Knotigova, P.,Duskova, J.,Dohnalek, J.,Masek, J.,Turanek, J.,Raska, M.,Maly, P.
Myomedin scaffold variants targeted to 10E8 HIV-1 broadly neutralizing antibody mimic gp41 epitope and elicit HIV-1 virus-neutralizing sera in mice.
Virulence, 12:1271-1287, 2021

PubMed Abstract: One of the proposed strategies for the development of a more efficient HIV-1 vaccine is based on the identification of proteins binding to a paratope of chosen broadly neutralizing antibody (bNAb) that will mimic cognate HIV-1 Env (glyco)protein epitope and could be used as potent immunogens for induction of protective virus-neutralizing antibodies in the immunized individuals. To verify this "non-cognate ligand" concept, we developed a highly complex combinatorial library designed on a scaffold of human myomesin-1 protein domain and selected proteins called Myomedins specifically binding to variable regions of HIV-1 broadly neutralizing antibody 10E8. Immunization of mice with these Myomedin variants elicited the production of HIV-1 Env-specific antibodies. Hyperimmune sera bound to Env pseudotyped viruses and weakly/moderately neutralized 54% of tested clade A, B, C, and AE pseudotyped viruses variants . These results demonstrate that Myomedin variants have the potential to mimic Env epitopes and could be used as potential HIV-1 vaccine components.

PubMed: 33993840
DOI: 10.1080/21505594.2021.1920251

実験手法

X-RAY DIFFRACTION (1.8 Å)