6T3L

PAS-GAF fragment from Deinococcus radiodurans phytochrome in dark state

6T3L の概要

• エントリーDOI: 10.2210/pdb6t3l/pdb
• 分子名称: Bacteriophytochrome, 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid (3 entities in total)
• 機能のキーワード: kinase, photosensor, transferase, phytochrome
• 由来する生物種: Deinococcus radiodurans
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 75630.25

構造登録者

Claesson, E.,Takala, H.,Yuan Wahlgren, W.,Pandey, S.,Schmidt, M.,Westenhoff, S. (登録日: 2019-10-11, 公開日: 2020-04-08, 最終更新日: 2024-11-13)

主引用文献

Claesson, E.,Wahlgren, W.Y.,Takala, H.,Pandey, S.,Castillon, L.,Kuznetsova, V.,Henry, L.,Panman, M.,Carrillo, M.,Kubel, J.,Nanekar, R.,Isaksson, L.,Nimmrich, A.,Cellini, A.,Morozov, D.,Maj, M.,Kurttila, M.,Bosman, R.,Nango, E.,Tanaka, R.,Tanaka, T.,Fangjia, L.,Iwata, S.,Owada, S.,Moffat, K.,Groenhof, G.,A Stojkovic, E.,A Ihalainen, J.,Schmidt, M.,Westenhoff, S.
The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser.
Elife, 9:-, 2020

PubMed Abstract: Phytochrome proteins control the growth, reproduction, and photosynthesis of plants, fungi, and bacteria. Light is detected by a bilin cofactor, but it remains elusive how this leads to activation of the protein through structural changes. We present serial femtosecond X-ray crystallographic data of the chromophore-binding domains of a bacterial phytochrome at delay times of 1 ps and 10 ps after photoexcitation. The data reveal a twist of the D-ring, which leads to partial detachment of the chromophore from the protein. Unexpectedly, the conserved so-called pyrrole water is photodissociated from the chromophore, concomitant with movement of the A-ring and a key signaling aspartate. The changes are wired together by ultrafast backbone and water movements around the chromophore, channeling them into signal transduction towards the output domains. We suggest that the observed collective changes are important for the phytochrome photoresponse, explaining the earliest steps of how plants, fungi and bacteria sense red light.

PubMed: 32228856
DOI: 10.7554/eLife.53514

実験手法

X-RAY DIFFRACTION (2.07 Å)